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Original Research Article | OPEN ACCESS

Regulation of MicroRNA-378 expression in mature human adipose tissue cells by adiponectin, free fatty acids and dexamethasone

Lulian Xu, Li Jiang, Kefeng Gu, Zhenzhen Liu, Xu Xu

For correspondence:-  Xu Xu   Email: jg9048@163.com

Accepted: 20 October 2017        Published: 31 January 2018

Citation: Xu L, Jiang L, Gu K, Liu Z, Xu X. Regulation of MicroRNA-378 expression in mature human adipose tissue cells by adiponectin, free fatty acids and dexamethasone. Trop J Pharm Res 2018; 17(1):29-34 doi: 10.4314/tjpr.v17i1.5

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effects of adiponectin (ADPN), free fatty acids (FFAs), growth hormone (GH), and dexamethasone (DEX) on miR-378 expression in human adipose tissue cells, and their influence on regulation of obesity and insensitivity to insulin.
Methods: Human pre-adipocytes were cultured and differentiated. Adipocytes were treated with ADPN, FFAs, GH and DEX. RNA was isolated and quantified by real-time polymerase chain reaction (RT-PCR).
Results: Stimulation with FFA led to significant up-regulation of the expression of MiR-378 (approximately 3.8-fold) relative to control at the 4th hour (p < 0.01) in human mature adipose tissue cells. The expression of MiR-378 was increased almost 1.5-fold by ADPN within 24 h, relative to untreated control (p < 0.05).
Conclusion: The results of this study demonstrate that miR-378 expression is influenced by FFAs, ADPN, and DEX, the interaction of which may be involved in the pathogenesis of obesity-induced insensitivity to insulin. Thus, miR-378 is a potential biomarker for predicting the risk of complications, especially insulin resistance in obesity
 

Keywords: MiR-378, Adipocytes, Adiponectin, Free fatty acids, Growth hormone, Dexamethasone, Obesity, Insulin resistance

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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