Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gas chromatography-mass spectrometric analysis of nematicidal essential oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) roots and its activity against Heterodera avenae

Yun Chao Li, Hua Ji, Hong Tao Tao Li

For correspondence:- Hong Tao Li Email: lihongtao1314@hotmail.com Tel:+8631113933107622

Received: 25 March 2015 Accepted: 25 July 2015 Published: 29 September 2015

Citation: Li YC, Ji H, Li HT. Gas chromatography-mass spectrometric analysis of nematicidal essential oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) roots and its activity against Heterodera avenae. Trop J Pharm Res 2015; 14(9):1673-1678 doi: 10.4314/tjpr.v14i9.18

© 2015 The authors.
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Abstract

Purpose: To investigate the chemical composition and nematicidal activity of the essential oil of Valeriana amurensis roots against cereal cyst nematodes (Heterodera avenae).

Methods: The essential oil of V. amurensis roots was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). The nematicidal activity of the essential oil and its major constituents was determined against second stage juveniles of H. avenae.

Results: A total of 33 components of the essential oil were identified. The major constituents were bornyl acetate (12.5 %), patchoulol (11.6 %), caryophyllene (8.2 %), 3-methylvaleric acid (7.3 %) and isovaleric acid (6.5 %). The essential oil exhibited nematicidal activity against H. avenae with a medium lethal concentration (LC₅₀) value of 311.6 µg/mL. The major constituents, isovaleric acid and 3-methylvaleric acid, exhibited nematicidal activity against H. avenae with LC₅₀ of 218.2 and 683.8 µg/mL, respectively.

Conclusion: The study indicates that the essential oil of V. amurensis roots and its two major constituents, isovaleric acid and 3-methylvaleric acid, have a potential to be developed to natural nematicides for the control of cereal cyst nematodes.

Keywords: Valeriana amurensis, Heterodera avenae, Nematicidal activity, Isovaleric acid, 3-Methylvaleric acid, Essential oil, Cereal cyst nematodes