Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

α-Glucosidase and α-amylase inhibition potentials of ten wild Mexican species of Verbenaceae

José Ávila-Reyes, Norma Almaraz-Abarca, Eli A Delgado Alvarado, Rene Torres-Ricario , Néstor Naranjo-Jiménez, Marcela V Gutierrez-Velazquez, Laura Stepanian González-Valdez, José N Uribe-Soto, Andres Vasavilbazo-Saucedo

Laboratorio de Biotecnología, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional â€“ CIIDIR, Instituto Politécnico Nacional IPN, CP 34220, Calle Sigma, 119, Durango, DGO, Mexico;

For correspondence:- Rene Torres-Ricario Email: rtorresr@ipn.mx

Accepted: 17 December 2018 Published: 31 January 2019

Citation: Ávila-Reyes J, Almaraz-Abarca N, Delgado Alvarado EA, Torres-Ricario R, Naranjo-Jiménez N, Gutierrez-Velazquez MV, et al. α-Glucosidase and α-amylase inhibition potentials of ten wild Mexican species of Verbenaceae. Trop J Pharm Res 2019; 18(1):31-36 doi: 10.4314/tjpr.v17i18.5

© 2019 The authors.
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Abstract

Purpose: To evaluate the inhibitory activity of 10 wild Verbenaceae species from Mexico against α-glucosidase and α-amylase.

Methods: Ethanol leaf extracts of 10 Verbenaceae species from Mexico were prepared. The inhibitory activity of the extracts against α-glucosidase and α-amylase was evaluated using enzymatic protocols. At least four serial diluted concentrations of each extract was used to calculate the half-maximal inhibitory concentration (IC₅₀).

Results: The 10 evaluated Verbenaceae species showed high α-glucosidase inhibition activity, but a low inhibitory effect on α-amylase. Aloysia gratissima (IC₅₀ = 0.122 mg/mL), Verbena carolina (IC₅₀ = 0.112 mg/mL), Bouchea prismatica (IC₅₀ = 0.122 mg/mL), Verbena menthiflora (IC₅₀ = 0.071mg/mL) and Priva mexicana (IC₅₀ = 0.032 mg/mL) exhibited the strongest inhibitory activities against α-glucosidase.

Conclusion: All the Verbenaceae species studied possess α-glucosidase inhibitory effect, with P. mexicana being the one with the strongest activity. These findings demonstrate the highs potential of these species as a source of natural antihyperglycemic agents for type 2 diabetes therapy.

Keywords: Hyperglycemic, Diabetes, ^5;-Glucosidase, ^5;-Amylase Verbenaceae, Aloysia gratissima, Bouchea prismatica, Priva mexicana