Heng Du1,
Jing Zhuang2,
Yaping Gui3,
Qiuyue Xu4,
Li Zhu5,
Le Gao1,
Jie Jiang5
1Department of Urology, Second Affiliated Hospital of Xinjiang Medical University, Xinjiang 860063;
2Department of Nephrology, People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi, 830000;
3Tongji Hospital Affiliated Tongji University in Shanghai, Shanghai, 200000;
4Nanjing University of Chinese Medicine, Nanjing 210023;
5Department of Urology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, 130021, China.
For correspondence:- Jie Jiang
Email: jiangjiemedcch@163.com
Accepted: 26 August 2018
Published: 30 September 2018
Citation:
Du H, Zhuang J, Gui Y, Xu Q, Zhu L, Gao L, et al.
1, 25-Dihydroxyvitamin D3 suppresses cell cycle progression and thus growth of prostate cancer cells by inducing expression of limb bud and heart development (LBH). Trop J Pharm Res 2018; 17(9):1733-1738
doi:
10.4314/tjpr.v17i9.7
© 2018 The authors.
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Abstract
Purpose: To investigate the function of limb bud and heart development (LBH) in 1α, 25-dihydroxyvitamin D3 (1,25D)-mediated inhibitory effect on proliferation of prostate cancer cells.
Methods: The inhibitory effect of 1,25D on growth and cell cycle progression of lymph node carcinoma of the prostate (LNCaP) cells was determined using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry, while the expression levels of LBH in response to treatment with 1,25D were determined by quantitative reverse-transcription PCR (qRT-PCR) and western blottingting. The expression levels of LBH in cells were down/up regulated by transfection with siRNA or overexpression plasmids, and then cell growth and cell cycles were measured using the CCK-8 assay, EdU assay, and flow cytometry. Finally, the growth inhibitory effect of 1,25D on LBH knockdown cells were determined using CCK-8 and EdU assays.
Results: Treatment with 1,25D arrested LNCaP cells in G0/G1 phase of cell cycle, suppressed the growth of the cells and induced the expression of LBH. Overexpression/knockdown of LBH in LNCaP cells suppressed/promoted cell growth and accumulated/decreased cells in the G0/G1 phase. Moreover, knockdown of LBH reversed the inhibitory effect of 1,25D on cell proliferation of LNCaP cells.
Conclusion: Inhibitory effect of 1,25D on cell cycle progression and cell proliferation might be via LBH.
Keywords: Cell proliferation, Limb bud and heart development, Prostate cancer, 1^5;, 25-Dihydroxyvitamin D3