Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

A Novel Biological Synthesis of Gold Nanoparticle by Enterobacteriaceae Family

Soheyla Honary , Eshrat Gharaei-Fathabad, Zahra Khorshidi Paji, Masumeh Eslamifar

Mazandaran University of Medical Sciences, School of Pharmacy, Sari, Iran;

For correspondence:- Soheyla Honary Email: shonary@mazums.ac.ir

Received: 5 April 2012 Accepted: 3 November 2012 Published: 13 December 2012

Citation: Honary S, Gharaei-Fathabad E, Paji ZK, Eslamifar M. A Novel Biological Synthesis of Gold Nanoparticle by Enterobacteriaceae Family. Trop J Pharm Res 2012; 11(6):887-891 doi: 10.4314/tjpr.v11i6.3

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To demonstrate eco-friendly biosynthesis of gold nanoparticles by Enterobacteriaceae.

Methods: Pure colonies of nine different bacteria from the Enterobacteriaceae family were separated from water and cultured in Luria Bertani broth medium. Their respective supernatants were examined for ability to produce gold nanoparticles. In this step, 1 mM solution of Gold(III) chloride trihydrate H(AuCl4) added to reaction matrices (supernatant) separately. The reaction was performed in a dark environment at 37 ºC. After 24 h, it was observed that the color of the solutions turned to dark purple from light yellow. The gold nanoparticles were characterized by UV-Visible spectroscopy, dynamic light scattering, scanning electron microscopy and Fourier transform infrared spectroscopy (FTIR) for yield, particle size, shape and presence of different functional groups, respectively. The nanoparticles were centrifuged and re-dispersed in double distilled water thrice to purify them for FTIR studies.

Results: The gold nanoparticles were fairly uniform in size, spherical in shape and with Z-average diameter ranging from 11.8 to 459 nm depending on the bacteria used. FTIR spectra revealed the presence of various functional groups in the gold nanoparticles which were also present in the bacterial extract.

Conclusion: The current approach suggests that rapid synthesis of nanoparticles would be feasible in developing a biological process for mass scale production of gold nanoparticles.

Keywords: Biosynthesis, Enterobacteriaceae, Gold nanoparticles