Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Acute and subacute toxicity study of Aucklandia lappa Decne seed oil

Chunlan Su¹, Xue Zhang², Peng Du¹, Ting Zeng¹, Qin Liang¹, Jia Ran¹, Tingting Li¹, Xian Yang¹

¹Engineering Research Center for Biotechnology of Active Substances, Ministry of Education/Department of Life Sciences, Chongqing Normal University; ²Department of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing 401331, PR China.

For correspondence:- Xian Yang Email: 464997677@qq.com Tel:+8615310776297

Accepted: 20 September 2021 Published: 31 October 2021

Citation: Su C, Zhang X, Du P, Zeng T, Liang Q, Ran J, et al. Acute and subacute toxicity study of Aucklandia lappa Decne seed oil. Trop J Pharm Res 2021; 20(10):2117-2125 doi: 10.4314/tjpr.v20i10.15

© 2021 The authors.
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Abstract

Purpose: To investigate the acute and subacute toxicity of Aucklandia lappa Decne. seed oil (ALDO) in mice and rats.

Methods: A single dose of 10 g ALDO/kg was administered to Kunming mice in an acute oral toxicity experiment. Their weight and feed consumption were recorded for 14 days to observe whether they had symptoms of poisoning and mortality. Sprague-Dawley (SD) rats were administered 0.89, 1.77 and 3.54 g/kg for 28 days, and symptoms of poisoning and mortality were monitored daily. Body weight, feed consumption, hematology, serum biochemical parameters, relative organ weight, and histopathology of the experimental and control groups were compared.

Results: The acute oral toxicity study revealed that there was no significant difference in the macroscopic results, including mortality, feed consumption and weight growth between the group dosed with 10 g ALDO/kg (p > 0.05) and the control group. In the subacute toxicity test, SD rats had a higher weight growth rate and feed utilization after doses of 0.89 g ALDO/kg (p < 0.01). However, compared with the control group (p > 0.05), there was also no significant difference in biochemical and hematological parameters, relative organ weight, or in macroscopic and histological features of both animal types. The electrolyte concentrations of Na and Cl increased at the doses of 1.77 and 3.54 g/kg (p < 0.01).

Conclusion: These results suggest that ALDO is relatively safe when administered orally to rats and provide a theoretical basis for the development of new food resources.

Keywords: Aucklandia lappa seed oil, Acute toxicity, Rodents, Subacute toxicity, Histological features, Hematological properties