Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Analgesic and Anti-inflammatory Profile of n-Hexane Fraction of Viola betonicifolia

Naveed Muhammad¹, Muhammad Saeed¹ , Syed Naeemuddin Gilani², Ikram ul Haq³, Haroon Khan¹

¹Department of Pharmacy University of Peshawar; ²Pharmacology Section, PCSIR, Laboratories Complex Peshawar; ³National Institute of Health (NIH), Islamabad, Pakistan.

For correspondence:- Muhammad Saeed Email: saeedrph2000@yahoo.com Tel:+009203232175686

Received: 18 October 2011 Accepted: 9 November 2012 Published: 13 December 2012

Citation: Muhammad N, Saeed M, Gilani SN, Haq Iu, Khan H. Analgesic and Anti-inflammatory Profile of n-Hexane Fraction of Viola betonicifolia. Trop J Pharm Res 2012; 11(6):963-969 doi: 10.4314/tjpr.v11i6.13

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the analgesic and anti-inflammatory activities of n-hexane extract of the whole plant of Viola betonicifolia Sm, family: Violaceace.

Methods: The n-hexane fraction of Viola betonicifolia (VBHF) was tested for its analgesic and anti-inflammatory activities (carrageenan-induced and histamine-induced edema models) in BALB/c mice.

Results: VBHF exhibited significant (p < 0.01) analgesic and anti-inflammatory activity at test doses of 100, 200 and 300 mg/kg. The analgesic effect of VBHF was dose-dependent in acetic acid pain model while the extract was a weak analgesic at the dose of 300 mg/kg in hot plate and tail immersion test. Diclofenac sodium and tramadol showed better analgesic properties to the extract. Analgesia was not antagonized by naloxone in the hot plate model. Anti-inflammatory activity against carrageenan-induced edema was 60.8 %; however, histamine-induced inflammation was not antogonised by the extract.

Conclusions: The extract has some analgesic and anti-inflammatory activities. This justifies its use in traditional medicine for pain of management.

Keywords: Viola betonicifolia, Analgesic, Anti-inflammatory