Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-Aging Activity and Non-Toxic Dose of Phytooxyresveratrol from Artocarpus lakoocha Roxb

Prasit Suwannalert¹ , Nasapon Povichit^2,3, Pranom Puchadapirom¹, Mutita Junking⁴

¹Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400; ²Detox (Thailand) Co. Ltd., Chiang Mai 50200; ³Youcando R&D Institute, Youcando Co Ltd., Bangkok 10510; ⁴Division of Medical Molecular Biology, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

For correspondence:- Prasit Suwannalert Email: parasit109@yahoo.com Tel:+6622015578

Received: 26 June 2011 Accepted: 17 January 2012 Published: 21 February 2012

Citation: Suwannalert P, Povichit N, Puchadapirom P, Junking M. Anti-Aging Activity and Non-Toxic Dose of Phytooxyresveratrol from Artocarpus lakoocha Roxb. Trop J Pharm Res 2012; 11(1):69-74 doi: 10.4314/tjpr.v11i1.9

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the anti-aging activity and toxicity doses of phytooxyresveratrol extracted from Artocarpus lakoocha Roxb.

Methods: Artocarpus lakoocha 100 g was extracted with 2 ml of 95 % ethanol to obtain phytooxyresveratrol (POV). Total phenolic content, as well as free radical scavenging and anti-glycation activities of POV were characterized in order to assess its anti-aging properties. The models of DNA nicking and bacterial reverse mutation (Ames) were applied to the extract in order to determine its effective and toxic doses, respectively.

Results: Phytooxyresveratrol (POV) exhibited antiaging activity. It also showed high levels of phenolic content, radical scavenging activity and anti-glycation. A POV concentration of 25 µg/ml promoted strong anti-DNA nicking. Furthermore, it was non-toxic at concentrations ranging from 5 - 100 µg/ml.

Conclusion: In vitro, phytooxyresveratrol (POV) extracted from Artocarpus lakoocha exerted effective anti-aging activity at a concentration of 25 µg/ml. Thus, the substance should be further studied for possible formulation into pharmaceutical products.

Keywords: Aging, Artocarpus lakoocha, Free radicals, Glycation, Phytooxyresveratrol, DNA nicking