Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-Thrombotic Effect of Carthamus tinctorius Linn Extracts in Rats

Sheng-hao Wu¹ , Cui-ping Zheng¹, Song-yan Chen¹, Xiao-ping Cai¹, Yue-jian Shi¹, Zhen Liu¹, Zhen-yu Li²

¹Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (Wenzhou Central Hospital), Wenzhou, Zhejiang, 325000; ²Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221000, China.

For correspondence:- Sheng-hao Wu Email: 546939322@qq.com Tel:+8615971479069

Received: 13 August 2013 Accepted: 22 September 2014 Published: 19 October 2014

Citation: Wu S, Zheng C, Chen S, Cai X, Shi Y, Liu Z, et al. Anti-Thrombotic Effect of Carthamus tinctorius Linn Extracts in Rats. Trop J Pharm Res 2014; 13(10):1637-1642 doi: 10.4314/tjpr.v13i10.10

© 2014 The authors.
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Abstract

Purpose: To explore the effects of Carthamus tinctorius L. (CTL) extracts on thrombosis in rats.

Methods: CTL extract was obtained in hot water (60 ^oC), dried in a hot air oven and then freeze-dried. The rats were divided into 6 groups: normal group, control group, reference group (aspirin 5 mg/kg) as well as groups that received 20, 40 and 80 mg/kg doses of CTL, respectively. For each group, treatment was given orally once daily for 14 days. Common carotid artery FeCl₃-induced thrombus and inferior vena cava thrombosis occlusion time, as well as plasma concentrations of thromboxane B2 (TXB2) and 6-keto-prostaglandine F1α(6-keto-PGF1α) were measured in rats.

Results: Compared with the control group, all doses of CTL extracts significantly and dose-dependently prolonged thrombosis occlusion time, reduced the weight of thrombus and increased inhibition rate (p < 0.01). Plasma TXB2 concentration of all CTL extracts groups decreased dose-dependently (p < 0.05) while that of 6-keto-PGF1α was increased (p < 0.05). There was association between 6-keto-PGF1α/TXB2 and arterial or venous thrombus weight for all treatments, and also with occlusion time for CTL treatment but not for aspirin.

Conclusion: CTL has a significant effect on thrombosis in rats. However, further studies are required to determine its clinical potentials.

Keywords: Carthamus tinctorius L., Thrombosis, Thromboxane B2, 6-Keto-prostaglandin F1^5;, Aspirin, Occlusion time