Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-Ulcerative Potential of Punica granatum L (Lythraceae) Hydroalcohol Fruit Peel Extract

Ghazaleh Moghaddam¹, Mohammad Sharifzadeh², Gholamreza Hassanzadeh³, Mahnaz Khanavi^4,2, Farzaneh Dolatshahi¹, Naficeh Sadeghi¹, Mohammad Reza Oveisi¹, Mannan Hajimahmoodi^1,5

¹Department of Drug and Food Control; ²Department of Pharmacology and Toxicology, Pharmaceutical Research Center, Faculty of Pharmacy; ³Department of Anatomy, School of Medicine; ⁴Department of Traditional Pharmacy, Faculty of Traditional Medicine; ⁵Department of Traditional Iranian Pharmacy Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

For correspondence:- Mannan Hajimahmoodi Email: hajimah@sina.tums.ac.ir

Received: 21 October 2013 Accepted: 9 May 2014 Published: 25 July 2014

Citation: Moghaddam G, Sharifzadeh M, Hassanzadeh G, Khanavi M, Dolatshahi F, Sadeghi N, et al. Anti-Ulcerative Potential of Punica granatum L (Lythraceae) Hydroalcohol Fruit Peel Extract. Trop J Pharm Res 2014; 13(7):1093-1097 doi: 10.4314/tjpr.v13i7.12

© 2014 The authors.
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Abstract

Purpose: To evaluate the antiulcer activities of the methanol extract of pomegranate (Punica granatum L) fruit peel using 80 % ethanol-induced gastric ulcer model in rats.

Methods: Male Wistar rats, 175 - 220 g, were fasted for 48 h, but had free access to water. They were randomly divided into nine experimental groups. Ulcer was induced in the rats with 80 % ethanol. The control group animals received the same treatment as those in the test groups except that the extract treatment was replaced by administration of appropriate volume of the dosing vehicle. Histamine-receptor type-2 (H2) blocker, cimetidine (100 mg/kg, i.p.) was used as the reference drug. Oral pretreatment with three different extract doses (25, 50 and 100 mg/kg) was for 15 days, thereafter, ulcer index (UI) and inhibition were calculated.

Results: The extract, at 50 mg/kg, of black fruit peel extract produced significant (p < 0.05) protective effect in rats with a preventive index of 65.87 %. Other doses were significantly protective against ethanol-induced gastric ulcer in the rats. On the other hand, north white peel was not effective (50 mg/kg) showed an ulcer index of 49.52 ± 1.99. Histopathological examination of the stomach of the ulcerated animals treated with white peel (50 mg/kg) showed severe erosion of gastric mucosa, sub-mucosal edema and neutrophil infiltration.

Conclusion: The study shows indicates the antiulcer properties of the methanol extracts of north white peel, sour summer and black peel (25, 50, 100 mg/kg) of pomegranate. Their antiulcer activity is exerted, possibly, via its high antioxidant activity.

Keywords: Anti-inflammatory, Pomegranate, Punica granatum, Peel extract