Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-hypertensive effect of Gastrodia elata Bl leaf extract in rats

Hua Fang, Wei-jing Zhang, Jing-chao Zhang, Miao Yang, Fang-xiang Zhang, Jian-ping Zhang

Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou Province, 550002, China;

For correspondence:- Jian-ping Zhang Email: succiniter@126.com Tel:+8685185921066

Received: 26 January 2017 Accepted: 12 July 2017 Published: 31 August 2017

Citation: Fang H, Zhang W, Zhang J, Yang M, Zhang F, Zhang J. Anti-hypertensive effect of Gastrodia elata Bl leaf extract in rats. Trop J Pharm Res 2017; 16(8):1887-1891 doi: 10.4314/tjpr.v16i8.19

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the probable antihypertensive effects of Gastrodia elata Bl. extract (GEBE) in renovascular hypertensive rats as well as the mechanism involved in blood pressure reduction.

Methods: The two-kidney one-clip (2K1C) Goldblatt model of renovascular hypertension was used in Wistar rats. The 2K1C group rats were treated with captopril (40 mg/kg/day), low-dose GEBE (150 mg/kg/day) and high-dose of GEBE (300 mg/kg/day) for 6 weeks by intragastric administration. Systolic (SBP) and diastolic blood pressure (DBP) were measured by the tail-cuff method. Urine creatinine and urea levels of the rats were measured by enzyme-linked immunosorbent assay (ELISA). Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were also evaluated.

Results: In the captopril- and GEBE-treated groups, blood pressure decreased progressively over the course of the 6-week treatment period compared with that of the untreated (control) rats (p < 0.01). High-dose GEBE also significantly increased plasma SOD activity but decreased plasma MDA concentration (p < 0.05). Renal function improved following captopril and GEBE (300 mg/kg/day) treatment (p < 0.01).

Conclusion: The results suggest that GEBE probably exerts an antihypertensive effect by inhibiting endothelin (ET)-converting enzyme and via its antioxidant activity.

Keywords: Antihypertensive, Gastrodia elata, Goldblatt renovascular hypertension, Endothelin-1, Hypertrophy