Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-inflammatory effect of date seeds (Phoenix dactylifera L) on carrageenan-induced edema in rats

Saryono Saryono¹, Warsinah Warsinah², Atyanti Isworo¹, Ferry Efendi³

¹School of Nursing; ²School of Pharmacy, Faculty of Health Sciences, University of Jenderal Soedirman; ³Faculty of Nursing, Universitas Airlangga, Surabaya, Indonesia.

For correspondence:- Ferry Efendi Email: ferry-e@fkp.unair.ac.id Tel:+62315913257

Accepted: 19 November 2018 Published: 27 December 2018

Citation: Saryono S, Warsinah W, Isworo A, Efendi F. Anti-inflammatory effect of date seeds (Phoenix dactylifera L) on carrageenan-induced edema in rats. Trop J Pharm Res 2018; 17(12):2455-2461 doi: 10.4314/tjpr.v17i12.22

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the anti-inflammatory properties and mechanism of action of date seed extract in carrageenan-induced edema in rats.

Methods: In this study with a pre- and post-test control group design, 30 Wistar rats were divided into six groups: Groups C1, C2, and C3 (given 1, 3, and 5 g/kg of soaked date seeds, respectively); positive control (PC, positive control given dexamethasone, 0.5 mg/kg dose), negative control (NC, edema induced by carrageenan), and healthy control (HC, no treatment). In groups C1–3, PC, and NC, carrageenan was administered intraperitoneally prior to date seed, PC and NC treatments. Prostaglandin E2 (PGE-2), cyclooxygenase (COX)-1, COX-2, interleukin (IL)-1β, and IL-12 were measured before and immdiate;y after treatment and compared between the groups.

Results: Steeped date seed modulated inflammatory mediators by decreasing IL-1β, COX-1, COX-2, IL-12, and PGE-2 significantly (p < 0.05). Its indicated that the steeped date seeds down-regulate pro-inflammatory mediators in a manner similar to that of dexamethasone as positive control.

Conclusion: Steeped date seeds possess anti-inflammatory activity similar to that of dexamethasone, through a mechanism involving decreases in pro-inflammatory mediators. Thus, date seeds is a potential source of herbal anti-inflammatory therapy.

Keywords: Phoenix dactylifera, Anti-inflammatory, Date seeds, Pro-inflammatory mediators, Dexamethasone