Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antidiabetic retinopathy effect of Fufang Danshen Mingmu in rats

Li Ma^1,2, Xiong Wang¹, Jin-hu Wu¹, An-lin Peng¹, Ling Liu³, Jie Jiang¹, Yong-gang Chen¹, Jing-sheng Xia¹

¹Department of Pharmacy, Tongren Hospital Affiliated to Wuhan University (The Third Hospital of Wuhan); ²Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430081; ³Department of ophthalmology, Tongren hospital affiliated to Wuhan University (The Third Hospital of Wuhan), Wuhan 430060, Hubei, China.

For correspondence:- Jing-sheng Xia Email: malia999@163.com Tel:+862768894991

Accepted: 18 October 2020 Published: 30 November 2020

Citation: Ma L, Wang X, Wu J, Peng A, Liu L, Jiang J, et al. Antidiabetic retinopathy effect of Fufang Danshen Mingmu in rats. Trop J Pharm Res 2020; 19(11):2395-2399 doi: 10.4314/tjpr.v19i11.21

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of Fufang Danshen Mingmu (FDM) on streptozotocin-induced diabetic retinopathy rats.

Methods: Diabetic retinopathy model rats were prepared using a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control group, control group, reference group (glibenclamide, 1 mg/kg) as well as FDM groups, (50, 100 and 200 mg/kg body weight). Blood glucose and plasma insulin levels were determined. Oxidative stress was evaluated in liver and kidney as lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats.

Results: Compared with diabetic rats, oral administration of FDM at a dose of 200 mg/kg daily for 30 days resulted in a significant decrease in fasting blood glucose (120.21 ± 3.37 mg/dL, p < 0.05) and increased insulin level (13.31 ± 0.67 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.86 ±0.24 mg/dL, p < 0.05) and serum urea (41.86±1.59 mg/dL, p < 0.05).

Conclusion: The results indicate that FDM normalizes impaired antioxidant status in streptozotocin-induced diabetic retinopathy rats, and also exerts a protective effect against lipid peroxidation by scavenging free radicals.

Keywords: Fufang Danshen Mingmu, Diabetic retinopathy, Antihyperglycemic, Antioxidant oxidative stress, Fasting blood glucose