Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidant, anti-inflammatory and antiulcer effects of Moroccan Ceratonia siliqua pulp in animal models

Soad Moubtakir¹, Chafik Terrafe¹, Mehdi Ait Laaradia^1,2, Majda Badaoui¹, Khadija Oubella¹, Fatima Zahra Agouram¹, Rachida Aboufatima³, Loubna El Yazouli¹, My AHmed EL Amiri⁴, Abderrahman Chait¹

¹Laboratory of Pharmacology, Neurobiology, Anthropology and Environment, Department of Biology, Faculty of Sciences, Semlalia, University Cadi Ayyad, BP 2390 40080 Marrakech, Morocco; ²ISPITS-Higher Institute of Nursing Professions and Health Techniques, Ministry of Health and Social Protection, Morocco; ³Laboratory of Genie Biologic, Sultan Moulay Slimane University, Faculty of Sciences and Technics, Beni-Mellal, Morocco; ⁴Laboratory of Biochemistry, Faculty of Pharmacology and Medicine, Cadi Ayyad, BP 2390 40080 Marrakech, Morocco.

For correspondence:- Abderrahman Chait Email: chafik.terrafe@edu.uca.ac.ma Tel:+212524434885

Accepted: 10 May 2024 Published: 29 May 2024

Citation: Moubtakir S, Terrafe C, Laaradia MA, Badaoui M, Oubella K, Agouram FZ, et al. Antioxidant, anti-inflammatory and antiulcer effects of Moroccan Ceratonia siliqua pulp in animal models. Trop J Pharm Res 2024; 23(5):847-853 doi: 10.4314/tjpr.v23i5.6

© 2024 The authors.
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Abstract

Purpose: To investigate the potential antioxidant, anti-inflammatory and antiulcer effects of methanol extract of Ceratonia siliqua (Fabaceae).

Methods: The methanol extract of Ceratonia siliqua pulp was obtained using Soxhlet apparatus. Xylene (20 µL) induced ear inflammation administered intraperitoneally and HCl/ethanol solution (1 mL) induced gastric ulceration administered intra-gastrically were used to induce inflammation and gastric ulcer, respectively. Twenty-four mice were randomly divided into 4 groups (n = 6). Furthermore, eighteen rats (Sprague Dawley) were divided into 4 groups. Control group received (10 mL/kg of saline), Ceratonia siliqua group received (250 and 500 mg/kg), and other groups received diclofenac sodium (10 mg/kg; p,o), and omeprazole (30 mg/kg; p,o). Phytochemical screening was carried out by following standard procedures. The percentage of inhibition, pH, and ulceration index were measured using pH metric titration and degree of ulceration. The antioxidant capacity was determined in vitro using radical DPPH scavenging and FRAP assays.

Results: Phytochemical screening reveals the presence of flavonoids, tannins, quinones and sterols. Acute toxicity assessment showed that methanolic extract of Ceratonia siliqua has a DL₅₀ higher than 5 g/kg. Oral administration of methanolic extract of Ceratonia siliqua (500 mg/kg) reduced significantly (p < 0.001) the percentage inflammation and ulceration index in both assays in comparison to control groups, these effects were comparable to those observed in reference drugs. The extract possessed a strong antioxidant capacity with low IC₅₀.

Conclusion: Ceratonia siliqua extract has antioxidant, anti-inflammatory and anti-ulcer properties. Standardization of Ceratonia siliqua extract will be required for the development of lead molecules against ulcers and inflammation.

Keywords: Ceratonia siliqua, Anti-inflammatory effect, Antioxidant activity, Antiulcer effect