Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidant potentials of five flavonoids compounds isolated from Varthemia iphionoids

Fatima Zerargui¹, Karima Saffidine¹, Thoraya Guemmaz¹, Haifaa Laroui¹, Hayat Trabsa² , Abderrahmane Baghiani¹, Seddik Khanouf³, Muza H Abu Zarga⁴

²Faculty of Exact Sciences and Nature and Life Sciences, University of Biskra, Biskra 07000, Algeria; ³Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif 1, Setif 19000, Algeria; ⁴Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif 1, Setif 19000, Algeria. ⁴Department of Chemistry, University of Jordan, Amman 11942, Jordan.

For correspondence:- Hayat Trabsa Email: hayat.trab@yahoo.fr Tel:+213675368146

Accepted: 20 June 2023 Published: 31 July 2023

Citation: Zerargui F, Saffidine K, Guemmaz T, Laroui H, Trabsa H, Baghiani A, et al. Antioxidant potentials of five flavonoids compounds isolated from Varthemia iphionoids. Trop J Pharm Res 2023; 22(7):1417-1425 doi: 10.4314/tjpr.v22i7.9

© 2023 The authors.
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Abstract

Purpose: To evaluate the in vitro antioxidant activity of some flavonoids isolated from Varthemia iphionoids aqueous methanol extract.

Methods: A portion (330 g) of the aqueous methanol extract of Varthemia iphionoids was adsorbed onto 200 g of S silica gel (70 - 230 mesh) and then loaded onto a column of S silica gel (70 - 230 mesh, 100 g). The column was packed with chloroform and eluted with chloroform-methanol mixtures of increasing polarity. Column chromatography purification (silica gel, 400 mesh) and thin-layer chromatography (TLC) or recrystallization yielded 5 compounds subsequently identified by nuclear magnetic resonance (NMR) and mass spectroscopy (MS).

Results: The antioxidant activities of five identified flavonoids: Kumatakillin (1), Penduletin (2), Jeceidine (3), 6-Methoxy isokaemfride (4), and 3,3’-Di-O-methyl quercetin (5) were determined. The results indicated that compound 3 exhibited the best scavenging activity against DPPH radical and H₂O₂ comparable to that of quercetin as standard. All compounds had the ability to prevent the oxidation of β-carotene. In anti-hemolytic assay, the five compounds gave HT₅₀ higher than vitamin C. The best protection activity of erythrocytes membrane was recorded with compound 2 (HT₅₀ = 60.14 ± 0.72 min). Moreover, all compounds had significantly increased blood clotting time, and among them, compounds 1, 2, and 3 exhibited significantly increased thrombolytic activity compared to the control (***p < 0.001).

Conclusion: These results indicate that Varthemia iphionoids has good antioxidant activities but further studies on the plant extract are required.

Keywords: Varthemia iphionoids, Flavonoids, NMR, Free radicals, Antioxidant, Antihemolytic, Anticoagulant