Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antiproliferative Effect of the Methanol Extract of Piper crocatum Ruiz & Pav Leaves on Human Breast (T47D) Cells In-vitro

Britanto Dani Wicaksono¹, Yohana Ayupriyanti Handoko², Enos Tangke Arung^1,2, Irawan Wijaya Kusuma², Dina Yulia¹, Aldrin Neilwan Pancaputra³, Ferry Sandra¹

¹Stem Cell and Cancer Institute, Jalan Ahmad Yani no. 2Pulomas, Jakarta, 13210; ²Wood Chemistry Laboratory, Forest Product Department, Forestry Faculty, Mulawarman University; Jalan KH. Dewantara, Kampus Gn. Kelua, Samarinda, East Borneo 75123; ³Dharmasis Cancer Hospital, Jalan Letjend S. Parman Kav 84-86, Jakarta 11420, Indonesia.

For correspondence:- Ferry Sandra Email: fsandra@sci-indonesia.org Tel:+622147860173

Received: 6 February 2009 Accepted: 14 April 2009 Published: 14 August 2009

Citation: Wicaksono BD, Handoko YA, Arung ET, Kusuma IW, Yulia D, Pancaputra AN, et al. Antiproliferative Effect of the Methanol Extract of Piper crocatum Ruiz & Pav Leaves on Human Breast (T47D) Cells In-vitro. Trop J Pharm Res 2009; 8(4):345-352 doi: 10.4314/tjpr.v8i4.9

© 2009 The authors.
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Abstract

Purpose: To investigate the anti-cancer properties of the methanol extract of Piper crocatum Ruiz & Pav. leaves and its mode of action in human breast cancer (T47D) cells.

Methods: The anti-cancer property and mechanism of action of the extract were evaluated by its effect on cell viability, nuclear morphology, cell cycle progression and the expression of phosphorylated p44/p42 as a marker for cell proliferation.

Results: The results showed that there was a reduction of cell viability by the extract in a concentration-dependent manner and no alteration of nuclear morphology was observed. There were negligible changes in Sub-G1 phase formation after extract treatment. expression of phosphorylated p44/42 was decreased by the extract only. Inclusion of the extract in the incubation medium decreased insulin-stimulated phosphorylation of p44/p42 indicating that the anti-proliferative effect of the extract was via p44/p42 pathway.

Conclusion: All together, the data indicate that P. crocatum methanol extract inhibits the growth of human breast cancer (T47D) cells via inhibition of p44/p42 phosphorylation.

Keywords: Piper crocatum leaf; methanol extract; antiproliferative; breast cancer; T47D cells