Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Benjakul supplementation improves hepatic fat metabolism in high-fat diet-induced obese rats

Achiraya Kamchansuppasin¹ , Kevalin Vongthoung², Pornthep Temrangsee¹, Narongsuk Munkong³, Nusiri Lerdvuthisopon⁴

¹Office of Graduate Studies, Faculty of Medicine, Thammasat University, Pathum Thani; ²Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok; ³Office Department of Medicine, School of Medicine, University of Phayao, Phayao; ⁴Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.

For correspondence:- Achiraya Kamchansuppasin Email: achiraya_chi@yahoo.com Tel:+66972671932

Accepted: 25 January 2020 Published: 30 April 2020

Citation: Kamchansuppasin A, Vongthoung K, Temrangsee P, Munkong N, Lerdvuthisopon N. Benjakul supplementation improves hepatic fat metabolism in high-fat diet-induced obese rats. Trop J Pharm Res 2020; 19(4):797-803 doi: 10.4314/tjpr.v19i4.17

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the effects of Benjakul water extract (BWE) supplementation for the prevention of hepatic fat metabolic dysfunction in a rat obesity model induced by a high-fat diet (HFD).

Methods: Forty male outbred Sprague-Dawley rats were separated into six groups according to diet composition and treatment: control, HFD, and HFD supplemented with Benjakul extraction at low and high dose (41.3 and 413 mg/kg/day, respectively). After 4 weeks, blood biochemical parameters (i.e., hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) enzyme and liver histological features) were examined. Subsequently, hepatic gene expression of sterol regulatory element binding protein-1 (SREBP-1) and nuclear factor-kappa B (NF-kB) were investigated.

Results: Low and high doses of BWE showed significant prevention of abdominal fat accumulation (p < 0.05) and inhibited hypercholesterolemia without restoring triglyceride (TG) and lipoprotein-cholesterol (LDL-C) in serum compared to rats fed HFD alone. BWE hindered hepatic fat accumulation via suppression of SREBP-1 expression and HMGCR activity in HFD-induced obese rats, while significantly promoting NF-kB down regulation (p < 0.05).

Conclusion: BWE may be a novel prophylactic strategy for preventing metabolic syndrome and to protect against steatosis due to its regulatory effects on lipid homeostasis.

Keywords: Benjakul, Fat metabolism, Non-alcoholic fatty liver disease, High-fat diet