Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Biological function and regulatory mechanism of mta2 expression in bladder cancerous cells

Xiaobo Guo , Gang Li, Yufeng Zhao, Zhao Bo

Department of Urology, Heji Hospital Affiliated with Changzhi Medical College, Changzhi 046011, Shanxi Province, China;

For correspondence:- Xiaobo Guo Email: tailuanci022988066@163.com

Accepted: 4 November 2023 Published: 30 November 2023

Citation: Guo X, Li G, Zhao Y, Bo Z. Biological function and regulatory mechanism of mta2 expression in bladder cancerous cells. Trop J Pharm Res 2023; 22(11):2247-2252 doi: 10.4314/tjpr.v22i11.1

© 2023 The authors.
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Abstract

Purpose: To study the function, regulation, and potential clinical use of mta2 gene in carcinoma of the bladder.

Methods: Viral vector and si-mta2 were transfected into both T24 and EJ bladder cancer cells, for induction of overexpression and knockdown of metastasis-associated gene 2 (mta2), respectively. In vitro invasiveness and migratory potential of cell lines were assayed using Matrigel and Transwell procedures, respectively, under the two mta2 expression conditions.

Results: Transwell migration data revealed that overexpressed mta2 protein significantly enhanced the migratory capacity of T24 bladder cancer cell line, while knockdown of mta2 significantly reduced the migration of EJ bladder cancer cell line (p < 0.01). Matrigel invasion assay showed that overexpression of mta2 protein significantly enhanced the invasive potential of T24 bladder cancer cell line, but knockdown of mta2 significantly (p < 0.01) reduced the invasive capacity of EJ bladder cancer cell line. There was a lower amount of E-cadherin protein in T24 bladder cancer cell line overexpressing mta2 than in cd511b-transfected and un-transfected cells, but N-cadherin protein level was significantly higher. Furthermore, there was a significantly higher amount of E-cadherin protein expression in EJ bladder cancer cell line with mta2 knockdown than in si-NC transfection and un-transfected cells (p < 0.01).

Conclusion: Knockdown of mta2 inhibits the proliferation, migration and invasion of bladder cancer cell lines through a mechanism related to the inhibition of epithelial-mesenchymal transition-related process proteins.

Keywords: mta2, Bladder cancer, Migration, Proliferation