Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Butein inhibits proliferation and migration of gastric cancer cells by regulating PI3K/AKT pathway

Kunhua Lin¹, Qiongjiao Lu², Shougang Wang³, Yina Lin¹, Tao Cui⁴

¹Department of Radiotherapy, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou City, Fujian Province 363000, China; ²Department of Radiation Oncology, Fujian Medical University, Fuzhou City, Fujian Province 350122, China; ³Office of Academic Affairs, Affiliated Hospital of Beihua University, Jilin City, Jilin Province, 132011, China; ⁴Department of Gastroenterology, Affiliated Hospital of Beihua University, Jilin City, Jilin Province 132011, China.

For correspondence:- Tao Cui Email: cuitao0818@163.com Tel:+8643262166238

Accepted: 25 November 2021 Published: 30 December 2021

Citation: Lin K, Lu Q, Wang S, Lin Y, Cui T. Butein inhibits proliferation and migration of gastric cancer cells by regulating PI3K/AKT pathway. Trop J Pharm Res 2021; 20(12):2545-2550 doi: 10.4314/tjpr.v20i12.13

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the possible effects of butein on the proliferation, apoptosis, and motility of gastric cancer cells, and the possible mechanism of action.

Methods: MTT assay was conducted to determine the effects of butein on gastric cancer cell proliferation. The effect of butein on gastric cancer cell apoptosis was evaluated by flow cytometry. In addition, wound closure and Transwell assays were performed to assess butein’s effects on gastric cancer cell motility, while immunoblot assays were performed to assess the effect of butein on the expression of proteins in the PI3K/AKT pathway of gastric cancer cells.

Results: Butein treatment suppressed gastric cancer cell proliferation and stimulated apoptosis (p < 0.001). In addition, butein treatment reduced the motility of gastric cancer cells, but mediated the PI3K/AKT axis and, hence, influenced the proliferation, apoptosis, and motility of gastric cancer cells (p < 0.001).

Conclusion: Butein inhibits the proliferation and migration of gastric cancer cells by regulating PI3K/AKT pathway. Thus, this compound has potentials for development as an agent for the treatment.

Keywords: Butein, Gastric cancer, Proliferation, Apoptosis, Cell migration, PI3K/AKT pathway