Kunhua Lin1,
Qiongjiao Lu2,
Shougang Wang3,
Yina Lin1,
Tao Cui4
1Department of Radiotherapy, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou City, Fujian Province 363000, China;
2Department of Radiation Oncology, Fujian Medical University, Fuzhou City, Fujian Province 350122, China;
3Office of Academic Affairs, Affiliated Hospital of Beihua University, Jilin City, Jilin Province, 132011, China;
4Department of Gastroenterology, Affiliated Hospital of Beihua University, Jilin City, Jilin Province 132011, China.
For correspondence:- Tao Cui
Email: cuitao0818@163.com Tel:+8643262166238
Accepted: 25 November 2021
Published: 30 December 2021
Citation:
Lin K, Lu Q, Wang S, Lin Y, Cui T.
Butein inhibits proliferation and migration of gastric cancer cells by regulating PI3K/AKT pathway. Trop J Pharm Res 2021; 20(12):2545-2550
doi:
10.4314/tjpr.v20i12.13
© 2021 The authors.
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Abstract
Purpose: To investigate the possible effects of butein on the proliferation, apoptosis, and motility of gastric cancer cells, and the possible mechanism of action.
Methods: MTT assay was conducted to determine the effects of butein on gastric cancer cell proliferation. The effect of butein on gastric cancer cell apoptosis was evaluated by flow cytometry. In addition, wound closure and Transwell assays were performed to assess butein’s effects on gastric cancer cell motility, while immunoblot assays were performed to assess the effect of butein on the expression of proteins in the PI3K/AKT pathway of gastric cancer cells.
Results: Butein treatment suppressed gastric cancer cell proliferation and stimulated apoptosis (p < 0.001). In addition, butein treatment reduced the motility of gastric cancer cells, but mediated the PI3K/AKT axis and, hence, influenced the proliferation, apoptosis, and motility of gastric cancer cells (p < 0.001).
Conclusion: Butein inhibits the proliferation and migration of gastric cancer cells by regulating PI3K/AKT pathway. Thus, this compound has potentials for development as an agent for the treatment.
Keywords: Butein, Gastric cancer, Proliferation, Apoptosis, Cell migration, PI3K/AKT pathway