Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cirsilineol inhibits the proliferation and migration of endometriotic cells

Xumeng Gu¹, Tianchan Zhang¹, Tongtong Liu², Huifang Cong¹, Wenpeng Wu³

¹Department of Gynaecology Ward 1, The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang Province 150001, China; ²Department of Gynecology and Obstetrics Ward, The Third Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang Province 150040, China; ³Department of Acupuncture Ward 3, The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang Province 150001, China.

For correspondence:- Wenpeng Wu Email: wuwenpeng3080701@163.com Tel:+8645187090221

Accepted: 25 November 2023 Published: 31 December 2023

Citation: Gu X, Zhang T, Liu T, Cong H, Wu W. Cirsilineol inhibits the proliferation and migration of endometriotic cells. Trop J Pharm Res 2023; 22(12):2427-2432 doi: 10.4314/tjpr.v22i12.2

© 2023 The authors.
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Abstract

Purpose: To determine the effect of cirsilineol on the proliferation and migration of endometriotic cells.

Methods: Human endometriotic epithelial cells 12Z were treated with 5, 10 and 20 μM cirsilineol, respectively. Cell proliferation was evaluated using BrdU incorporation assay whereas wound healing assay was used to investigate cell migration, and cell invasion was assessed by Transwell assay. Cell cycle was investigated using flow cytometry while protein expression and phosphorylation levels of p65 and IκBα were evaluated by Western blot.

Results: Treatment with cirsilineol at either 10 μM or 20 μM resulted in a significant reduction in cell proliferation and cell cycle arrest at the G2/M phase (p < 0.05). Moreover, cirsilineol weakened cell migration and invasion in 12Z cells, but did not alter the expression of p65. However, it significantly downregulated the phosphorylation of p65 and increased the expression of IκBα, while attenuating the phosphorylation level of IκBα (p < 0.05). Furthermore, 5 μM cirsilineol did not show any significant effects on these cellular activities, suggesting the activity of cirsilineol in 12Z cells is dose-dependent.

Conclusion: Cirsilineol inhibits cell proliferation, induces cell cycle arrest, suppresses migration and invasion of endometriotic epithelial cells by regulating NF-κB pathway. Thus, cirsilineol might be a potential therapeutic candidate for endometriosis treatment.

Keywords: Cirsilineol, Cell migration, Cell proliferation, Endometriotic cells, NF-?B pathway