Abd Almonem Doolaanea1,2
,
Nur Izzati Mansor1,
Nurul Hafizah Mohd Nor1,
Mohd Affendi Bin Mohd Shafri3,
Susi Sukmasari4,
Farahidah Mohamed1,2,5
1Department of Pharmaceutical Technology;
2IKOP Sdn. Bhd., Pilot Plant Pharmaceutical Manufacturing, Faculty of Pharmacy;
3Kulliyyah of Allied Health Sciences;
4Kulliyyah of Dentisry, International Islamic University Malaysia (IIUM), 25200 Kuantan;
5International Institute of Halal Research & Training (INHART), Kulliyyah of Engineering, IIUM, PO Box 10, 50728, Kuala Lumpur, Malaysia.
For correspondence:- Abd Doolaanea
Email: abdalmonemdoolaanea@yahoo.com Tel:+60136238628
Accepted: 23 September 2017
Published: 31 October 2017
Citation:
Doolaanea AA, Mansor NI, Nor NH, Shafri MA, Sukmasari S, Mohamed F.
Comparative assessment of poly (D,L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption. Trop J Pharm Res 2017; 16(10):2315-2324
doi:
10.4314/tjpr.v16i10.2
© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption.
Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection.
Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection.
Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.
Keywords: Chitosan, cetyltrimethyl ammonium bromide (CTAB), Nanoparticle, Poly (D,L-lactide-co-glycolide) PLGA, Plasmid DNA adsorption, Gene therapy