Bing-Mu Fang1,
Jin-Hong Jiang1,
Xue-Wu Zhang2,
Jian Fan2,
Shuang-shuang Li2,
Xiang-Min Tong2 
For correspondence:- Xiang-Min Tong Email: tongxiangmin@163.com Tel:+8657187236628
Received: 22 February 2014 Revised: 20 December 2014 Published: 28 February 2015
Citation:
Fang B, Jiang J, Zhang X, Fan J, Li S, Tong X.
Curcumin enhances bortezomib treatment of myeloma by inhibiting heat shock protein 90 ex
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate whether curcumin augments bortezomib-induced apoptosis in myeloma cells (MM1.R line), and to explore the molecular mechanism with regard to heat shock protein 90 (HSP90) ex
Methods: MTT cell viability assay was used to assess growth inhibition of MM1.R cells at different concentrations of curcumin alone and also combined with 0.01 mM bortezomib. Annexin V and propidium iodide (PI) labeling were used to detect apoptosis. Caspase 3, caspase 9, NF-κB, and HSP 90 protein ex
Results: Curcumin alone inhibited MM1.R cell growth and increased apoptosis in a concentration-dependent manner. When curcumin was combined with low concentration (0.01 mM) bortezomib, both effectsA288;viability inhibition and apoptosis induction increased (p < 0.05), whereas bortezomib alone had no effect (p > 0.05). Western blotting revealed that for curcumin and combined treatments, ex
Conclusion:Curcumin increased MM1.R cell sensitivity to bortezomib, which may be due to suppression of NF-κB and HSP90 activity.
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