Wu Jia-liang,
Ma Dan-wei 
,
Wang Ya-nan,
Zhang Hong,
He Bing,
Li Qun,
Zou Zhi-yan,
Feng Jing
For correspondence:- Ma Dan-wei Email: danwei10ma@163.com Tel:+8613699478376
Received: 17 April 2013 Accepted: 25 October 2013 Published: 24 December 2013
Citation: Jia-liang W, Dan-wei M, Ya-nan W, Hong Z, Bing H, Qun L, et al. Cytotoxicity of Essential Oil of Chenopodium ambrosioides L against Human Breast Cancer MCF-7 Cells. Trop J Pharm Res 2013; 12(6):929-933 doi: 10.4314/tjpr.v12i6.10
© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the cytotoxic activity of the essential oil of Chenopodium ambrosioides L. against human breast cancer MCF-7 cells.
Methods: Cytotoxicity was characterized by 50 % inhibition (IC50) of human breast cancer cell lines (MCF-7) using 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was analysed by Hoechst33258 staining and DNA ladder. MCF-7 cellular superoxide dismutase (SOD), catalase (CAT) vitality and malondialdehyde (MDA) content were evaluated.
Results: The essential oil was cytotoxic to MCF-7 cell line. A dose- and time-dependent inhibition was observed with IC50 values of 18.75, 9.45 and 10.50 μg/ml at 6, 24 and 48 h, respectively. Analyses by Hoechst33258 staining and DNA ladder indicate that the essential oil induced apoptosis. SOD vitality significantly decreased (pA308; 0.05) by 51 % when the concentration of the essential oil increased from 1.25 to 12.5 μg/ml while CAT vitality significantly increased (p A308; 0.05) by 71 % when essential oil concentration was similarly increased. The MDA content of each treatment group, when compare to control, did not show any significant difference (p A308; 0.05).
Conclusion: The essential oil of C. ambrosioides was cytotoxic to MCF-7 cell line and induced apoptosis.
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