Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Denatonium benzoate decreases the effect of histamine in vitro and in rats

Plamen I Zagorchev^1,2, Vesela Yu Kokova³ , Milena N Draganova-Filipova^2,4, Elisaveta G Apostolova³,

¹Department of Physics and Biophysics, Faculty of Pharmacy; ²Research Institute at Medical University-Plovdiv; ³Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy; ⁴Department of Medical Biology, Medical Faculty, Medical University-Plovdiv 15A Vasil Aprilov Blvd, 4002 Plovdiv, Bulgaria.

For correspondence:- Vesela Kokova Email: vesela_uk@abv.bg Tel:+35932602099

Accepted: 26 August 2020 Published: 30 September 2020

Citation: Zagorchev PI, Kokova VY, Draganova-Filipova MN, Apostolova EG, Denatonium benzoate decreases the effect of histamine in vitro and in rats. Trop J Pharm Res 2020; 19(9):1879-1885 doi: 10.4314/tjpr.v19i9.12

© 2020 The authors.
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Abstract

Purpose: To evaluate the effect of denatonium benzoate (DB) in histamine-induced model of inflammation and the effect of the selective H1 receptor agonist (2-(2-Pyridyl) ethylamine) on rat gastric smooth muscle strips pretreated with DB.

Methods: The anti-inflammatory effect of DB was evaluated in vivo on histamine-induced rat paw edema. In vitro studies on spontaneous muscle contraction were performed on smooth muscle strips isolated from rat gastric corpus.

Results: The results showed a well-defined anti-inflammatory effect of DB (15 mg/kg) during the early stage of rat paw edema at the 15th (p < 0.001), 30th (p < 0.01) and 60th min (p < 0.001) compared to control. In vitro experiments indicated reduced spontaneous contractile activity of smooth muscle strips to H1 receptor agonist in the presence of DB (0.5 μM). The vascular effects of histamine are mediated by H1 receptors. Substances, which reduce the effect of histamine on the H1 receptors could influence the early stage of histamine-induced inflammation.

Conclusion: The results show that the anti-inflammatory activity of DB probably is related to its antagonistic activity on histamine H1 receptors. The results would contribute to the search for new anti-inflammatory drugs.

Keywords: Denatonium benzoate, Inflammation, Histamine, Muscle contraction