Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Determination of dehydroepiandrosterone in dietary supplements by reversed-phase HPLC

Yahia Z Tabaza¹ , Kamal M Mansi², Hanan A Azzam³, Farah F Al-Mamoori¹, Ali M Al-Samyda⁴, Talal A Aburjai¹

¹Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942; ²Department of Medical Laboratory Science, Faculty of Science, Al al-Bayt University, PO Box 130040, Mafraq 25113; ³Hamdi Mango Center for Scientific Research, The University of Jordan, Amman 11942, Jordan; ⁴Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan.

For correspondence:- Yahia Tabaza Email: y.tabaza@ju.edu.jo Tel:+96265355000

Accepted: 16 January 2021 Published: 28 February 2021

Citation: Tabaza YZ, Mansi KM, Azzam HA, Al-Mamoori FF, Al-Samyda AM, Aburjai TA. Determination of dehydroepiandrosterone in dietary supplements by reversed-phase HPLC. Trop J Pharm Res 2021; 20(2):383-387 doi: 10.4314/tjpr.v20i2.24

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To develop a reversed phase high performance liquid chromatography (HPLC) method for the determination of dehydroepiandrosterone (DHEA) in dietary supplements.

Methods: A reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of DHEA in dietary supplements. An isocratic system consisting of methanol and water (70:30 v/v) was run at a flow rate of 1 mL/min on a C18 HPLC column to achieve the separation. The method was validated with regard to linearity, intra-day and inter-day precision, and limits of both detection and quantification.

Results: The method achieved a retention time of 10.8 min, a resolution of 4.12, a detection limit (LOD) of 50 ng/μL, a quantification limit (LOQ) of 166.7 ng/μL and a label claim of 108.6 % with a relative standard deviation (RSD) of 0.38 % over a range of 0.0625 – 0.50 mg/mL with a correlation coefficient of 0.9997.

Conclusion: The method is simple, cost effective, time-saving and reliable for determining DHEA when compared to other reported methods in literature. Thus, it will be of benefit to manufacturers of this dietary supplement to adopt the method for quantitative laboratory analysis.

Keywords: Dehydroepiandrosterone, Prasterone, Dietary supplement, HPLC, Method development, Validation