Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Development and Application of Ligand-Exchange Reaction Method for the Determination of Clonazepam

Snezana S Mitić, Aleksandra N Pavlović , Snezana B Tosić, Emilija T Pecev, Milan N Mitić, Milan B Stojković

University of NiÅ¡, Faculty of Sciences and Mathematics, Department of Chemistry, ViÅ¡egradska 33, P.O.Box 224, 18000 NiÅ¡, Serbia;

For correspondence:- Aleksandra Pavlović Email: pavlovican@yahoo.com Tel:+38118533015

Received: 16 April 2011 Accepted: 9 December 2011 Published: 21 February 2012

Citation: Mitić SS, Pavlović AN, Tosić SB, Pecev ET, Mitić MN, Stojković MB. Development and Application of Ligand-Exchange Reaction Method for the Determination of Clonazepam. Trop J Pharm Res 2012; 11(1):91-98 doi: 10.4314/tjpr.v11i1.12

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: This paper presents an improved kinetic-spectrophotometric procedure for determining clonazepam (CZP) in pharmaceutical formulations and human serum.

Methods: The method is based on ligand-exchange reaction. The reaction was followed spectrophotometrically by measuring the rate of change of absorbance at 425 nm in ethanolic sodium hydroxide solution.

Results: The optimum operating conditions for reagent concentrations and temperature were established. Linear calibration curve was obtained in the range of 0.32 - 4.10 µg mL^-1. The optimized conditions yielded a theoretical detection limit of 0.24 µg mL^-1 based on the 3.3S_ocriterion, where S₀ is standard deviation of the calibration line. The interference of certain drugs, foreign ions and amino acids on the reaction rate were studied in order to assess the selectivity of the method.

Conclusion: The developed method is sensitive, accurate and reproducible and could be used for routine anlysis of clonazepam in pharmaceutical preparations and serum samples.

Keywords: Clonazepam, Ligand-exchange reaction, Kinetic spectrometry, Validation, Pharmaceutical preparation, Human serum