A Alper Öztürk1,2
,
Lucia Martin Banderas2,
Maria D. Cayero Otero2,
Evrim Yenilmez1,
Behiye Senel3,
Yasemin Yazan1
1Department of Pharmaceutical Technology, Faculty of Pharmacy, Anadolu University, Eski#1;ehir, Turkey;
2Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Seville, Seville, Spain;
3Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Anadolu University, Eski#1;ehir, Turkey.
For correspondence:- A Öztürk
Email: aaozturk@anadolu.edu.tr Tel:+90223350580
Accepted: 16 November 2018
Published: 31 January 2019
Citation:
Öztürk AA, Banderas LM, Otero MD, Yenilmez E, Senel B, Yazan Y.
Dexketoprofen trometamol-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles: Preparation, in vitro characterization and cyctotoxity. Trop J Pharm Res 2019; 18(1):1-11
doi:
10.4314/tjpr.v18i1.1
© 2019 The authors.
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Abstract
Purpose: To design, formulate and characterize sustained-release formulations of dexketoprofen trometamol (DT) nanoparticles (NPs)
Methods: Dexketoprofen trometamol (DT)-loaded poly(lactic-co-glycolic acid) (PLGA) NPs were produced by double emulsion-solvent evaporation method. The NPs were variously characterized for drug loading and release, particle profile, as well as by thermal analysis, x-ray difraction (XRD), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance analysis (1H-NMR). Furthermore, the NPs were evaluated for cytotoxicity against NIH-3T3 cells by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Results: The DT-loaded NPs demonstrated nanostructural characteristics and extended drug release. Particle size was in the range of 243 and 295 nm which remained unchanged in drug stability testing in simulated gastrointestinal media. Encapsulation efficiency ranged from 49 – 64 % for all the formulations. Higuchi and Korsmeyer-Peppas were the best-fit release kinetic models for the NPs containing 5 and 10 % DT, respectively. The NPs with 10 % DT presented no significant cytotoxicty at the doses and periods studied.
Conclusion: Stable and non-toxic DT NPs with potential for sustained and controlled release of the drug have been successfully developed.
Keywords: Dexketoprofen trometamol, Poly-lactic-co-glycolic acid (PLGA), Nanoparticles, Release kinetics, Stability