Shibiao Chen1,
Weicheng Liu1,
Yanping Zhan1,
Ranran Xu1,
Zhang Hao1,
Jichun Liu2 
For correspondence:- Jichun Liu Email: liujichun999@gmail.com Tel:+8697188692707
Received: 12 January 2015 Accepted: 18 May 2015 Published: 29 June 2015
Citation: Chen S, Liu W, Zhan Y, Xu R, Hao Z, Liu J. Dietary cholesterol protects anesthesia-induced cognitive deficits in Wistar rats. Trop J Pharm Res 2015; 14(6):1035-1040 doi: 10.4314/tjpr.v14i6.14
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the effect of cholesterol on frequent exposure of anesthesia-induced cognitive impairment in wistar rats.
Methods: Healthy wistar rats were divided in two groups, the gp I rats fed with regular diet and gp II with cholesterol diet. These groups were further divided into sub-groups as gp Ia (n=8) and gp IIa (n=8). These sub-groups received weekly exposure of anesthesia for 6 weeks. Animals were anesthetized by subcutaneous sodium thiopental injection. Cortical nerve growth factor levels were measured by indirect sandwich enzyme-linked immunosorbent assay (ELISA) while total protein was determined by Bradford protein assay.
Results: Group IIa (cholesterol-fed animals) as well as Group IIb (cholesterol-fed followed by anesthesia) showed significant increase in body weight (25 to 50 g, p < 0.03), but no such increase was observed in other groups. However, group Ib showed a significant (43.07 %, p F6; 0.001) decrease in the level of nerve growth factor when compared with group Ia. Moreover, significantly decreased cytokines IL-1β levels (59.09 %, p < 0.005) and TNF-α (20 %, p < 0.025) of group IIa more effectively than in group Ia rats. Microglial marker showed significantly increase (16.66 %, p < 0.025) in cholesterol diet group. Overall increase in leakage of anti-rat IgG (blood brain barrier marker) was found in both groups (IIa and IIb).
Conclusion: The results suggest that dietary cholesterol protects or neutralizes anesthesia-induced cognitive deficits in rats.
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