Jian-Bo Zhou,
Gang Peng 
,
Jia Wang,
Shu Nie,
Jun Li,
Yu-Chen Jia,
Qiu-Ying Zhang
For correspondence:- Gang Peng Email: gangpeng_gs@126.com
Received: 15 May 2015 Accepted: 5 September 2015 Published: 31 October 2015
Citation: Zhou J, Peng G, Wang J, Nie S, Li J, Jia Y, et al. Ebracteolatain A and ebracteolatain B induce apoptosis of human hepatoma cell line (HepG2). Trop J Pharm Res 2015; 14(10):1821-1828 doi: 10.4314/tjpr.v14i10.12
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the effects of ebracteolatain A (EA) and ebracteolatain B (EB) from Euphorbia ebracteolata Hyata (Euphorbiaceae) on the proliferation of HepG2 cells and the possible mechanisms.
Methods: EA and EB from E. ebracteolata were obtained by column chromatography. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays were used to study the cytotoxic and pro-apoptotic activities of EA and EB against HepG2 cells. Western blot assay was used to investigate the possible mechanisms of action.
Results: EA and EB were successfully isolated from E. ebracteolata by column chromatography. The results of MTT assay indicate that EA and EB have significant anti-proliferative activities against HepG2 cells in dose- and time-dependent manners with half maximal inhibitory concentration (IC50) of 28.48 and 31.72 μg/mL. respectively. The results of flow cytometry assay suggest that EA and EB significantly (p < 0.01) induced the apoptosis of HepG2 cells at the levels of 47.45 and 42.26 %, respectively. Western blot data indicate that EA and EB significantly (p < 0.05 or 0.01) down-regulated the ex
Conclusion: EA and EB inhibit the proliferation of HepG2 cells, the probable mechanisms being associated with mitochondria-mediated apoptosis.
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