Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Camphor on the Behavior of Leukocytes In vitro and In vivo in Acute Inflammatory Response

Saulo Euclides Silva-Filho, Francielli Maria de Souza Silva-Comar, Luiz Alexandre Marques Wiirzler, Rilson Jose do Pinho, Renata Grespan, Ciomar Aparecida Bersani-Amado, Roberto Kenji Nakamura Cuman ,

Laboratory of Inflammation, Department of Pharmacology and Therapeutics, State University of Maringa, Maringa, PR, Brazil;

For correspondence:- Roberto Cuman Email: rkncuman@uem.br Tel:+55 44 3011-4867

Received: 12 May 2014 Accepted: 26 October 2014 Published: 15 December 2014

Citation: Silva-Filho SE, Silva-Comar FM, Wiirzler LA, do Pinho RJ, Grespan R, Bersani-Amado CA, et al. Effect of Camphor on the Behavior of Leukocytes In vitro and In vivo in Acute Inflammatory Response. Trop J Pharm Res 2014; 13(12):2031-2037 doi: 10.4314/tjpr.v13i12.13

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the effect of camphor on acute inflammatory response by leukocytes on chemotaxis, antiedematogenic and phagocytic activities.

Methods:The effect of camphor in acute inflammatory response evaluated by neutrophil chemotaxis, ear edema induced by croton oil, activity of the enzyme myeloperoxidase (MPO) and phagocytic activity of macrophages in Swiss mice.

Results: Camphor treatment did not show any cytotoxicity. Camphor at 3, 10, and 30 µg/ml doses exhibited significant (p < 0.01) reduction on leukocyte migration toward N-formyl methionyl leucyl phenylalanine fMLP. Topical treatment with camphor did not reduce significant ear edema or MPO activity at any of the doses tested. However, in contrast, oral treatment with 100 and 200 mg/kg camphor significantly (p < 0.01) reduced ear edema and myeloperoxidase (MPO) activity. Additionally, the phagocytic activity of macrophages was not affected by camphor.

Conclusions: These results indicate that the anti-inflammatory activity of camphor may be related to the inhibition of leukocyte migration and antiedematogenic activity.

Keywords: Camphor, Inflammatory response, Chemotaxis, Macrophages, Phagocytic, Leukocytes, Edema