Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Carvacrol on Salmonella Saintpaul Biofilms on Stainless Steel Surface

Nancy Sayuri Uchida¹, Renata Grespan², Michelle Piovezan¹, Erika C Ferreira¹, Miguel Machinski Junior³, Roberto Kenji Nakamura Cuman², Jane Martha G Mikcha¹

¹Graduate Program in Health Sciences, Department of Clinical Analysis and Biomedicine; ²Graduate Program in Health Sciences, Department of Pharmacology and Therapeutics; ³Graduate Program in Health Sciences, Department of Basic Health Sciences, State University of Maringa, Maringa, Brazil.

For correspondence:- Jane Mikcha Email: jmgmikcha@uem.br Tel:+55 (44) 3011-4813

Received: 19 December 2013 Accepted: 12 November 2014 Published: 15 December 2014

Citation: Uchida NS, Grespan R, Piovezan M, Ferreira EC, Junior MM, Cuman RK, et al. Effect of Carvacrol on Salmonella Saintpaul Biofilms on Stainless Steel Surface. Trop J Pharm Res 2014; 13(12):2021-2025 doi: 10.4314/tjpr.v13i12.11

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the effect of carvacrol against Salmonella saintpaul biofilms on stainless steel surface.

Methods: The effect of carvacrol on planktonic cells of S saintpaul was evaluated by determining the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The action of carvacrol on S. saintpaul biofilms on stainless steel surface was evaluated on established biofilm and on biofilm formation by counting the number of bacterial cells that adhered to the surface and by scanning electron microscopy.

Results: The antimicrobial activity of carvacrol against planktonic cells of S. saintpaul was observed. The highest inhibitory effect of carvacrol was observed during biofilm formation at 78 and 117 μg ml^-1 when S. saintpaul biofilms were reduced by 3 log cycles (p < 0.05).

Conclusion: Carvacrol reduced the number of bacterial cells that adhered to stainless steel surface, making it a potential compound for Salmonella saintpaul control.

Keywords: Salmonella spp., Biofilm, Carvacrol, Antimicrobial activity, Planktonic cells, Stainless steel