Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Elephantopus Scaber Linn leaf extract on mouse immune system

Yufri Aldi , Megaraswita , Dwisari Dillasamola

Department of Pharmacology, Faculty of Pharmacy, Andalas University, Padang, Indonesia;

For correspondence:- Yufri Aldi Email: yufrialdi@phar.unand.ac.id Tel:+6285274558508

Accepted: 20 September 2019 Published: 31 October 2019

Citation: Aldi Y, M, Dillasamola D. Effect of Elephantopus Scaber Linn leaf extract on mouse immune system. Trop J Pharm Res 2019; 18(10):2045-2050 doi: 10.4314/tjpr.v18i10.7

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose:To investigate the effect of Elephantopus Scaber Linn. ethanol extract on mouse immune system based on macrophage phagocytic activity and capacity, total leukocyte count, and distribution of each type of leukocyte.

Methods: Twenty male mice were randomly assigned to four groups. Group I (control) received sodium carboxy methyl cellulose (Na-CMC), 0.5 %). Groups II, III, and IV were dosed orally with 10, 30, and 100 mg/kg ethanol E. scaber leaf extract, respectively, for 7 consecutive days. On the 8th day, a suspension of Staphylococcus aureus was injected intraperitoneally, and macrophage activity and capacity as well as leukocyte count were then measured using a counting chamber device (hemocytometer) and a 400 x microscope.

Results: The effect of the leaf extract at doses of 10, 30, and 100 mg/kg was increase in macrophage activity by 46.25, 54, and 65 %, and macrophage capacity by 95.8, 105.4 and 125.8 cell/100 macrophage, respectively; total leukocyte count was 10790, 12360, and 15230 cells/µl, respectively, and lymphocyte was 34.4, 34.6, and 36 %. Thus, the leaf extract significantly increased macrophage activity and capacity, as well as segmented neutrophils, and mice total leukocyte counts (p < 0.05).

Conclusion: Ethanol extract of E scaber leaf extract increases macrophage activity and capacity, as well as total leukocyte count in mouse; therefore, this extract may provide a highly effective approach for boosting immunity.

Keywords: Leukocytes, Macrophages, Phagocytosis, Elephantopus Scaber Linn