Dawood Ahmad Hamdani1 
,
Aqeel Javeed1,
Muhammad Ashraf1,
Jawad Nazir2,
Aamir Ghafoor3
For correspondence:- Dawood Hamdani Email: davidbhai@hotmail.com Tel:+924299211103
Received: 26 August 2014 Accepted: 21 October 2014 Published: 24 November 2014
Citation: Hamdani DA, Javeed A, Ashraf M, Nazir J, Ghafoor A. Effect of Ketoprofen on Immune Cells in Mice. Trop J Pharm Res 2014; 13(11):1809-1813 doi: 10.4314/tjpr.v13i11.6
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To study the immunosuppressant and immunopotentiating effects of ketoprofen on antibody-producing cells.
Methods: Mice were given ketoprofen at doses of 1 mg/kg/day and 5 mg/kg/day for seven days. Similarly polyinosinic–polycytidylic acid (Poly IC) and phosphate buffer saline (PBS) were used as positive and negative control, respectively, for seven days. After seven days, the mice were sacrificed and their spleens removed. Simultaneously, blood was withdrawn from the hearts of the mice and serum was separated from the blood. The spleen cells were analyzed by enzyme-linked immunospot (ELISPOT) while the serum was investigated by enzyme-linked immunosorbant (ELISA) to evaluate the effects of ketoprofen on the ability of individual cell to produce antibodies and antibody- mediated immune responses.
Results: Ketoprofen significantly (p < 0.001) reduced the ability of individual cells to produce antibodies. There was a significant difference (p < 0.001) in % of spot forming cells of PBS treated negative control group (0.045 %) as against positive control (0.058 %), 1 mg ketoprofen /kg/day (0.037 %) and 5 mg ketoprofen/kg/day (0.032 %) treated groups. The results of ELISA showed a significant (p < 0.005) difference in the absorbance values between negative control, positive control, ELISA control and ketoprofen treated groups. Absorbance was significantly (p < 0.005) reduced in ketoprofen-treated groups.
Conclusion: The ability of an individual cell to produce antibodies and antibody-mediated immune responses is suppressed by ketoprofen, suggesting that it is immunosuppressive, and thus indicating its potential application in patients with auto-immune disorders.
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