Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Lonicerae japonicae caulis extract on the growth and apoptosis of human osteosarcoma U2OS cells

XiaoWei Dong, RuShan Yan, QiJun Yao, Yu Lei Haidong Liang

Department of Bone and Soft Tissue Repair and Reconstructive Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, China;

For correspondence:-

Accepted: 24 July 2024 Published: 31 August 2024

Citation: Dong X, Yan R, Yao Q, Lei Y.Liang H. Effect of Lonicerae japonicae caulis extract on the growth and apoptosis of human osteosarcoma U2OS cells. Trop J Pharm Res 2024; 23(8):1255-1260 doi: 10.4314/tjpr.v23i8.4

© 2024 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the effect of Lonicerae japonicae Caulis extract (LJCE) on the proliferation, apoptosis, and cycle distribution of human osteosarcoma U2OS cells.

Methods: Human osteosarcoma U2OS cells were treated with LJCE at doses of 10, 20, 30 and 40 mg/mL, with untreated U2OS cells as the control group. Cell proliferation was measured using MTT method, while flow cytometric analysis was used for determination of apoptosis and cell cycle distribution.

Results: As LJCE concentration increased, the viability of U2OS cells gradually decreased, while the apoptosis rate gradually increased. The LJCE treatment had no marked effect on G1 phase cells (p > 0.05). However, the population of G2 cells in each group increased with increasing LJCE concentration, while the S phase cells in each group decreased with increasing concentration. The cells were significantly blocked in the G2 phase (p < 0.05).

Conclusion: Lonicerae japonicae Caulis extract inhibits the proliferation of U2OS cells and promotes apoptosis of U2OS cells. Therefore, LJCE possesses potentials as a novel therapeutic agent for osteosarcoma.

Keywords: Lonicerae japonicae Caulis extract, U2OS, Apoptosis, Proliferation