Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Mongolian medicine alcohol spray therapy on fracture healing in rabbits

Qilatu A, Rihan Wu, Rilige Su

Osteopathic Hospital Doctor Office, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, China;

For correspondence:- Rilige Su Email: 17648551718@163.com

Accepted: 28 November 2023 Published: 31 December 2023

Citation: A Q, Wu R, Su R. Effect of Mongolian medicine alcohol spray therapy on fracture healing in rabbits. Trop J Pharm Res 2023; 22(12):2459-2465 doi: 10.4314/tjpr.v22i12.6

© 2023 The authors.
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Abstract

Purpose: To evaluate the healing effect and mechanism of Mongolian medicine alcohol spray therapy on fracture healing process in rabbits.

Methods: Thirty New Zealand white rabbits were randomly divided into normal, fracture, and treatment groups, with 10 rabbits in each group. Fracture and treatment groups underwent bone-fracture modeling, with the latter receiving daily alcohol spraying and stroking for six weeks. Serum calcium and phosphorus were measured using an automatic biochemical analyzer. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of BMP-2, ALP, and TGF-β. Biomechanical testing was performed to assess callus properties, and real-time polymerase chain reaction (PCR) and western blot were performed to assess BMP-2, Runx2, and p-Smad1/5 expressions in callus.

Results: There were low serum calcium, phosphorus, BMP-2, ALP, and TGF-β levels in fracture and treatment groups, compared to normal group. Similarly, treatment group showed significantly increased levels of serum calcium, phosphorus, bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), and transforming growth factor-β (TGF-β) (p < 0.05) compared to fracture group. Biomechanical testing revealed that maximum load, stiffness, and deflection of callus in the fracture and treatment groups were significantly lower compared to normal group (p < 0.05). expressions of BMP-2, Runx2, and p-Smad1/5 mRNA in the callus significantly decreased in both fracture and treatment groups compared to normal group (p < 0.05). However, treatment group showed significantly increased expression levels compared to fracture group (p < 0.05).

Conclusion: Mongolian medicine alcohol spray therapy promotes fracture healing in rabbits and increases maximum load of the callus, possibly through upregulation of the BMP-2 signaling pathway. Further research is necessary to confirm the exact mechanism by which alcohol spray therapy regulates BMP-2 signaling pathway.

Keywords: Mongolian medicine, Alcohol spray therapy, Fracture, Bone Morphogenetic Protein 2, Transforming Growth Factor-β