Alireza Feizbakhsh1,
Haniyeh Pazoki1,
Vahid Mohammadrezaei1,
Mohammad Ali Ebrahimzadeh2 
For correspondence:- Mohammad Ebrahimzadeh Email: zadeh20@yahoo.com Tel:+9815135430813
Received: 14 June 2013 Accepted: 2 February 2014 Published: 23 April 2014
Citation: Feizbakhsh A, Pazoki H, Mohammadrezaei V, Ebrahimzadeh MA. Effect of Phytohormones on the Composition of Sambucus ebulus Leaf Essential Oil. Trop J Pharm Res 2014; 13(4):581-586 doi: 10.4314/tjpr.v13i4.14
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the effect of growth hormones - naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) - on the essential oil of Sambucus ebulus leaf.
Methods: The leaves of S. ebulus were sprayed three times in one week with distilled water (as control) or with a solution of either NAA or IAA (150 ppb). Following the treatment, the leaves were collected from each of the plant and dried in the dark in a dry environment. The essential oil content of the leaves was obtained by hydrodistillation and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS).
Results: Sixty constituents were identified in the plant oil, some of which could have been responsible for the plant’s biological and/or toxicological activities. The results indicate that NAA and IAA exerted significant effect on the composition of the essential oil, increasing some components and decreasing some others significantly. In some cases, certain compounds were eliminated completely from the oil.
Conclusion: The use of phytohormones seems a useful strategy for modifying the composition of the essential oil in plants.
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