Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Zaoxiu ointment on wound-healing in experimental second degree burns rats

Xiong Wang¹, Li Ma¹, Meng-ling Huang², Yong-gang Chen¹, Jin-hu Wu¹

¹Department of Pharmacy, Tongren Hospital of Wuhan University (The Third Hospital of Wuhan), Wuhan 430060; ²College of Pharmacy, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, China.

For correspondence:- Jin-hu Wu Email: wujinhu133@126.com Tel:+862768894852

Accepted: 25 August 2019 Published: 30 September 2019

Citation: Wang X, Ma L, Huang M, Chen Y, Wu J. Effect of Zaoxiu ointment on wound-healing in experimental second degree burns rats. Trop J Pharm Res 2019; 18(9):1913-1917 doi: 10.4314/tjpr.v18i9.19

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the healing efficacy of a Traditional Chinese Medicine (TCM) preparation Zaoxiu Burn Ointment (ZBO) on experimental burns in rats.

Methods: The rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and ZBO-treated group. ZBO, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. The observation of wound area contraction and histological analysis of wound tissues were performed. The effect of ZBO on MMPs, VEGF and Type-III collagen proteins of wound tissue in rats were determined by Western blot.

Results: After 10 days of topical treatment with ZBO, ZBO-treated group showed faster reduction in wound area in comparison with control groups (p < 0.01). MMP-2, MMP-9, VEGF and type-III collagen expression of the wound tissue increased significantly (p < 0.05) in comparison with the control burn wounds in rats. The ZBO had no cytotoxic effect on BHK-21 cell line. Histological results showed an overall early recovery and regeneration in ZBO-treated group when compared with control group.

Conclusion: ZBO possesses significant wound-healing activity in full-thickness burn wounds in rats, and can potentially be developed for the treatment of burns patient in future.

Keywords: Zaoxiu burn ointment, Second-degree burns, wound-healing