Phanit Koomhin1,2,
Chuchard Punsawad1,
Prasit Suwannalert3,
Sarawoot Palipoch2
1School of Medicine;
2The Pathobiology of Cell and Tissue Research Group, Walailak University, Nakhon Si Thammarat, 80161;
3Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
For correspondence:- Sarawoot Palipoch
Email: sarawoot.pa@wu.ac.th Tel:+6675672873
Received: 16 November 2016
Accepted: 7 April 2017
Published: 30 May 2017
Citation:
Koomhin P, Punsawad C, Suwannalert P, Palipoch S.
Effect of a heme oxygenase-1 inducer on NADPH oxidase expression in alcohol-induced liver injury in male Wistar rats. Trop J Pharm Res 2017; 16(5):1039-1044
doi:
10.4314/tjpr.v16i5.10
© 2017 The authors.
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Abstract
Purpose: To investigated the effect of hemin, a heme oxygenase-1 (HO-1) inducer, on nicotinamide adenine dinucleotide phosphate oxidase (NOX) expression in rats with alcohol-induced liver injury.
Methods: Male Wistar rats were randomly divided into four groups consisting of the control group, the ethanol (EtOH) group, the EtOH + zinc protoporphyrin IX (ZnPP-IX) group and EtOH + hemin group. Hepatic NOX gene expression and immunohistochemistry of hepatic NOX1 and NOX4 were investigated in week 4.
Results: EtOH significantly increased levels of NOX. An immunohistochemical study demonstrated a high number of immunopositive hepatocytes for NOX1 in the EtOH group and EtOH + ZnPP-IX group compared with the control group. Hemin administration downregulated NOX gene expression and lowered the number of immunopositive hepatocytes for NOX1. In contrast, ZnPP-IX (HO-1 inhibitor) administration caused upregulation of NOX gene expression and increased the number of immunopositive hepatocytes for NOX1.
Conclusion: HO-1 inducer, hemin, alleviates oxidative stress-induced alcoholic liver injury by reducing NOX, especially NOX1
Keywords: NADPH oxidase, Immunohistochemistry, Heme oxygenase-1, Hemin, Reactive oxygen species, Alcohol-induced liver disease