Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of carvacrol on Salmonella Saintpaul biofilms on stainless steel surface

Nancy S Uchida¹, Renata Grespan², Michelle Piovezan¹, Erika C Ferreira¹, Miguel Machinski Júnior³, Roberto KN Cuman², Jane MG Mikcha¹

¹Graduate Program in Health Sciences, Department of Clinical Analysis and Biomedicine; ²Graduate Program in Health Sciences, Department of Pharmacology and Therapeutics; ³Graduate Program in Health Sciences, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil.

For correspondence:- Jane Mikcha Email: jmgmikcha@uem.br Tel:+554430114813

Received: 11 May 2014 Accepted: 20 April 2015 Published: 29 June 2015

Citation: Uchida NS, Grespan R, Piovezan M, Ferreira EC, Júnior MM, Cuman RK, et al. Effect of carvacrol on Salmonella Saintpaul biofilms on stainless steel surface. Trop J Pharm Res 2015; 14(6):1075-1079 doi: 10.4314/tjpr.v14i6.20

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the effect of carvacrol against Salmonella Saintpaul biofilms on stainless steel surface.

Methods: The effects of carvacrol on planktonic cells were evaluated by determining the minimum inhibitory concentration and minimal bactericidal concentration. The action of carvacrol on Salmonella Saintpaul biofilms on stainless steel surface was evaluated on established biofilm and on biofilm formation by counting the number of bacterial cells that adhered to the surface using scanning electron microscopy.

Results: The antimicrobial activity of carvacrol in planktonic cells of Salmonella Saintpaul was observed. The highest inhibitory effect of carvacrol was observed on biofilm formation at different sub-inhibitory concentrations.

Conclusion: Carvacrol reduced the number of bacterial cells that adhered to stainless steel surface, making it a potential compound for Salmonella Saintpaul control.

Keywords: Salmonella spp, Biofilm, Planktonic cells, Carvacrol, Stainless steel