Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of doxycycline-bioglass treatment on calvarial bone defect in rats: A histological study

Mona Mokhtarian¹, Mohammad Reza Nourani², Nasrin Esfahanizadeh¹

¹Department of Periodontics, Islamic Azad University of Tehran, Faculty of Dentistry; ²Division of Tissue Engineering, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

For correspondence:- Nasrin Esfahanizadeh Email: n_esfahanizadeh@yahoo.com Tel:+989122156687

Accepted: 18 May 2020 Published: 30 June 2020

Citation: Mokhtarian M, Nourani MR, Esfahanizadeh N. Effect of doxycycline-bioglass treatment on calvarial bone defect in rats: A histological study. Trop J Pharm Res 2020; 19(6):1243-1248 doi: 10.4314/tjpr.v19i6.19

© 2020 The authors.
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Abstract

Purpose: To evaluate the osteogenic properties of a bioglass-doxycycline complex for bone regeneration applications in calvarial bone defects in rats.

Methods: Three critical-size bone defects were created in each of eight experimental rats using a trephine bur. The experimentally created defects were then filled with bioglass-doxycycline (BG-D group), bioglass alone (BG group), or left unfilled (control group). Four randomly selected rats were sacrificed after 4 weeks while the other four rats were sacrificed after 8 weeks. The amount of newly regenerated bone and the osteoblast and osteoclast counts were calculated using histological analysis.

Results: Increase in the amount of regenerated bone was significant in the bioglass-doxycycline group at both 4 and 8 weeks (p < 0.05). Differences between the three groups in the mean number of osteoblasts were also significant at both 4 and 8 weeks (p < 0.05). Comparison of the three groups revealed significant increase in osteogenesis rate and considerable enhancement of the number of osteoblasts in both bioglass and bioglass-doxycycline groups (p < 0.05).

Conclusion: The complex of doxycycline and bioglass increased the rate of osteogenesis and number of osteoblasts in rats but decreased the number of osteoclasts. Therefore, bioglass-doxycycline can be considered as a bone graft in bone regeneration for medical applications.

Keywords: Bone, Regeneration, Osteoblasts, Osteoclasts, Osteogenesis, Bioglass, Doxycycline