Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of erythropoietin on microvascular anastomosis in rat

Sadık Sentürk¹ , Zeynep Karacor², Zekeriya Tosun², Adem Ozkan³, M Cihat Avunduk⁴, Murat Zumrut⁵

¹Mevlana (Rumi) University Hospital; ²Selcuk University Hospital; ³Department of Plastic and Reconstructive Surgery, Pamukkale University Hospital; ⁴Department of Pathology, Selcuk University Hospital; ⁵Department of Orthopedics and Traumatology, Mevlana (Rumi) University Hospital, Konya, Turkey.

For correspondence:- Sadık Sentürk Email: ssenturk@mevlana.edu.tr Tel:+44442001115

Received: 15 May 2015 Accepted: 1 August 2015 Published: 29 September 2015

Citation: Sentürk S, Karacor Z, Tosun Z, Ozkan A, Avunduk M, Zumrut M. Effect of erythropoietin on microvascular anastomosis in rat. Trop J Pharm Res 2015; 14(9):1629-1634 doi: 10.4314/tjpr.v14i9.12

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the re-endothelialization potential of erythropoietin (EPO) following microvascular anastomosis in rat femoral artery.

Methods: Ninety-six male Sprague-Dawley rats weighing between 300 g and 320 g were allocated randomly into two groups (control and EPO, n = 48). Left femoral artery microvascular anastomosis was performed in the rats. The control group was given 0.5 mL of 0.09 % NaCl subcutaneously 48 h post-operatively while the EPO group was given 150 U/kg recombinant human erythropoietin subcutaneously 48 h post-operatively. Tissue was harvested from each group after 1, 3, 5, and 7 days. All of the rats were sacrificed for morphometric analysis. Each arterial segment was analyzed morphometrically using Clemex Image Analysis software by a single examiner. The cross-sectional area of the media and neointima was measured and the intima/media ratio was calculated. Hematocrit measurement was carried out for all the rats.

Results: The I/M ratio differed significantly (p < 0.05) between the control and erythropoietin groups, at all time-points. The hematocrit was significantly (p < 0.05) lower in the control group than in the erythropoietin groups on day 7, but not on the other days.

Conclusion: This study demonstrates that erythropoietin markedly inhibits neointima formation with accelerated re-endothelialization in rat femoral arteries following microvascular anastomosis.

Keywords: Erythropoietin, Microvascular anastomosis, Re-endothelialization, Hematocrit, Neointima/media ratio