Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of hydrogen sulfide on PC12 cell injury induced by high ATP concentration

Kunli Yang^1,2, Hu Luo¹, Yajie Liu³, Yankai Ren¹, Meixia Guo¹, Bin Wang¹, Chih-huang Yang³ Dongliang Li^1,4

¹Sanquan College of Xinxiang Medical University, Xinxiang 453003; China; ²Department of Physiology, North Sichuan Medical College, Nanchong 637007, China; ³Ineye Hospital of Chengdu University of TCM, 610084, China; ⁴Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 453003, China.

For correspondence:- Dongliang Li Email: yklphy101@126.com

Accepted: 29 January 2022 Published: 28 February 2021

Citation: Yang K, Luo H, Liu Y, Ren Y, Guo M, Wang B, et al. Effect of hydrogen sulfide on PC12 cell injury induced by high ATP concentration. Trop J Pharm Res 2022; 21(2):281-288 doi: 10.4314/tjpr.v21i2.10

© 2022 The authors.
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Abstract

Purpose: To investigate the potential protective effect of hydrogen sulfide against neural cell damage induced by a high-concentration of adenosine triphosphate (ATP).

Methods: PC12 cells were incubated with ATP in order to induce cell damage. The extracellular level of H₂S and protein expression of cystathionine-β-synthase (CBS) were determined. The PC12 cells pre-treated with NaHS, aminooxyacetic acid (AOAA) and KN-62, prior to further incubation with ATP, and the effect of the treatments on cell viability was investigated.

Results: High-concentration ATP induced cell death in PC12 cells, and this was accompanied by markedly increased contents of extracellular H₂S and CBS expression (p < 0.05). The ATP-induced cytotoxicity was significantly compromised after pretreatment with H₂S. (p < 0.05). The viability of PC12 cells pretreated with NaHS and AOAA was significantly higher than that of PC12 cells treated with ATP alone. In addition, the viability of ATP-treated PC12 cells was further markedly increased after pretreatment with NaHS and KN-62 (p < 0.05).

Conclusion: ATP induced a concentration- and time-dependent cytotoxicity in PC12 cells via the endogenous H₂S/CBS system. Supplementation with exogenous H₂S mitigated the cell damage induced by high concentration of ATP via a specific mechanism which may be specifically related to P2X7R.

Keywords: Hydrogen sulfide, Adenosine triphosphate, Purinergic P2X7 Receptor, Cell damage, Cystathionine-?-synthase, Neural cells