Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells

Hua-Li Shi, Xiao-Feng Yang , Xin-Wei Li, Zhuo-Ling Fu, Xue-Qing Jin, Hai-Lian Ji

Department of Medicine, Hangzhou Medical College, Hangzhou 310053, Zhejiang, PR China;

For correspondence:- Xiao-Feng Yang Email: xfyhzmc@163.com

Received: 20 December 2016 Accepted: 14 June 2017 Published: 31 July 2017

Citation: Shi H, Yang X, Li X, Fu Z, Jin X, Ji H. Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells. Trop J Pharm Res 2017; 16(7):1595-1600 doi: 10.4314/tjpr.v16i7.18

© 2017 The authors.
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Abstract

Purpose: To investigate the effect of polygonimitin C (PC) on bone formation and resorption in human osteoblast-like MG63 cells.

Methods: MG63 cells were treated with PC at doses of 0, 20, 40 or 80 μg/mL for 48 h, with an untreated group as control. The effect of PC on alkaline phosphatase (ALP) activity in MG63 cells was investigated by p-nitrophenyl phosphate disodium hexahydrate assay. Western blot assay was used to evaluate the effect of PC on the expressions of osterix (OSX), bone morphogenetic protein-2 (BMP-2), runt-related transcription factor 2 (RUNX-2), osteocalcin (OC), fibronectin (FN), type I collagen (COL I), osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL) proteins in MG63 cells.

Results: ALP relative activity in MG63 cells treated with PC at 20, 40 or 80 μg/mL (123.58, 137.74 or 159.62 %, respectively) was significantly (p < 0.05 or 0.01) higher than that in control group (99.37 %). expressions of OSX, BMP-2, RUNX-2, OC, FN, COL I and OPG proteins in MG63 cells treated with PC at 20, 40 or 80 μg/mL were significantly (p < 0.01) higher than those in control group. However, there were no statistically significant differences in RANKL protein expression between PC-treated MG63 cells and control group.

Conclusion: These results show that PC exerts protective effects against osteoporosis by promoting bone formation and inhibiting bone resorption. Thus, PC may be useful in the development of new anti-osteoporosis drugs.

Keywords: Polygonimitin C, MG63 cells, Bone formation, Bone resorption, Osteoporosis