Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of total flavonoids from Drynaria rhizome on bone loss in ovariectomized rats

Fang Yu¹, QingNa Lv², ZhiHong Tong², WenJi Song², ZhengNan Zhao², Bo Yuan², HaiDong Liang²

¹The Second Hospital of Dalian Medical University, Dalian 116023; ²Hands and Feet Microsurgery, The Second Hospital of Dalian Medical University, Dalian 116023, People's Republic of China.

For correspondence:- HaiDong Liang Email: lianghddlf@sina.com

Accepted: 27 May 2019 Published: 30 June 2019

Citation: Yu F, Lv Q, Tong Z, Song W, Zhao Z, Yuan B, et al. Effect of total flavonoids from Drynaria rhizome on bone loss in ovariectomized rats. Trop J Pharm Res 2019; 18(6):1285-1289 doi: 10.4314/tjpr.v18i6.20

© 2019 The authors.
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Abstract

Purpose: To determine the potential effect of total flavonoids from Drynaria rhizome on bone loss in ovariectomized (OVX) rats.

Methods: The rats were divided into four groups: normal control, ovariectomized (OVX) control, and two Drynaria rhizome (DR) flavonoids treatments. Post-operation, osteoporotic OVX rats were given Drynaria rhizome total flavonoids for 3 months. Thereafter, the expressions of bone-related genes and biochemical indices were investigated in samples taken from rat serum and bone.

Results: Treatment with total flavonoids from Drynaria rhizome prevented bone mineral loss and improved some related biochemical indices associated with osteoporosis, namely, alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP), bone gla protein (BGP) and estradiol (E2). Reverse transcription-polymerase chain reaction (RT-PCR) data showed that treatment with the total flavonoids significantly downregulated mRNA expression of Wnt10b, β-catenin, recombinant human bone morphogenetic protein-2 (BMP2) and BMP4 in OVX rats, but significantly reversed OVX-induced downregulation of dickkopf1 (Dkk1) mRNA expression.

Conclusion: These results indicate that total flavonoids from Drynaria rhizome exert anti-osteoporotic effects in rats through the WNT signaling and BMP-2 signaling pathways.

Keywords: Drynaria rhizome, Bone loss, Total flavonoids, Ovariectomized rats, BMP-2 signaling pathway