Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effects of SOCS3 on the development of colon cancer via regulation of HIF-1α

Fang Chen¹, Yifan Long², Cuiyun Guo², Hui Zou³, Yingying Wang³, Kunbo Dong³

¹Department of Spleen and Stomach, Yantai Hospital of Traditional Chinese Medicine, Yantai, China; ²Department of Gastroenterology, The 970th Hospital of the PLA Joint Logistics Support Force l, Yantai, China; ³Department of Endoscopy, Yantai Hospital of Traditional Chinese Medicine, Yantai, China.

For correspondence:- Kunbo Dong Email: dkbdym1@163.com Tel:+8618253541913

Accepted: 30 June 2022 Published: 31 July 2022

Citation: Chen F, Long Y, Guo C, Zou H, Wang Y, Dong K. Effects of SOCS3 on the development of colon cancer via regulation of HIF-1α. Trop J Pharm Res 2022; 21(7):1391-1396 doi: 10.4314/tjpr.v21i7.5

© 2022 The authors.
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Abstract

Purpose: To investigate the influence of suppressor of cytokine signaling 3 (SOCS3) on rats with colon cancer (CC).

Methods: Sprague-Dawley (SD) rats were randomly divided into CC group and control group, and then CC rat model was constructed. The expression of SOCS3 in CC tissues was determined by quantitative real time-polymerase chain reaction (qRT-PCR). Hematoxylin-eosin staining (H&E) was used to examine colon tissue morphology. Immunohistochemistry (IHC) staining assay was performed to determine the expression of SOCS3 protein in colon tissues. The contents of HIF-1α, phosphorylated phosphatidylinositol 3-hydroxy kinase (p-PI3K), and phosphorylated protein kinase B (p-AKT) proteins were determined by Western blotting (WB).

Results: Compared with that in the control group, the number of tumors in CC group was significantly increased (p < 0.05). 2). On the other hand, protein and message ribonucleic acid (mRNA) expressions of SOCS3 were down-regulated in CC group (p < 0.05). 3), while protein expressions of p-PI3K, p-AKT and HIF-1α were raised in CC group (p < 0.05).

Conclusion: SOCS3 is lowly expressed in CC rats, and promotes the expression of HIF-1α by activating PI3K/AKT signaling pathway. Thus, SOCS3 provides a therapeutic strategy for the management of colon cancer.

Keywords: Colon cancer, Suppressor of cytokine signaling protein 3 (SOCS3), Hypoxia inducible factor-1α