Firdevs Kahvecioglu1 
,
Hayriye Esra Ülker2
1Department of Pedodontics, Faculty of Dentistry, Selcuk University, Konya, Turkey;
2Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkey.
For correspondence:- Firdevs Kahvecioglu
Email: drfirdevskahveci@hotmail.com
Accepted: 15 March 2022
Published: 30 April 2022
Citation:
Kahvecioglu F, Ülker HE.
Effects of herbal-based toothpastes on cell viability. Trop J Pharm Res 2022; 21(4):795-799
doi:
10.4314/tjpr.v21i4.16
© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To evaluate the effects of herbal-based toothpastes with different contents on the viability of L929 cells.
Methods: Herbal-based toothpastes {Aloe vera - propolis herbal toothpaste, Group 1; Parodontax, Group 2; Toothpaste with miswak, propolis and tea tree extract, Group 3, Dent natural protective toothpaste with clay, Group 4; Gano fresh, Group 5} were diluted (1:1, 1:2, 1:4, 1:8, 1:16, and 1:32) in Dulbecco’s modified Eagle’s medium. L929 fibroblast cells were treated with the medium containing the herbal toothpastes for 2 min. Cell viability was assessed using methyl tetrazolium test. The viability of the negative control group was set at 100 %, and the percentage viability of all groups was determined accordingly.
Results: Cell viability was significantly reduced at all dilutions in Group 5 (p = 0.00). This trend was also observed in Group 4. All dilutions except 1:32 significantly affected cell viability (p = 0.00). In Group 2, only the 1:1 dilution showed a toxic effect (p = 0.00). The samples in Groups 1 and 3 did not show statistically significant cytotoxicity to L929 cells (p > 0.05).
Conclusion: Herbal toothpastes containing substances such as sodium lauryl sulfate (Group 5) and sodium benzoate (Groups 2 and 4) are cytotoxic towards L929 cells. Groups 1 and 3 did not contain detergents but contain potassium sorbate as a preservative; hence, they are not toxic.
Keywords: Cytotoxicity, Herbal toothpaste, Sodium lauryl sulfate, Sodium benzoate, Potassium sorbate
Fulltext in PDF
