Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Emodin induces osteosarcoma cell apoptosis by promoting ATM protein cleavage

Ran Liao¹, Jianfeng Cao²

¹Department of Pharmacy, Affiliated Nanhua Hospital, University of South China, Hengyang 421002, Hunan Province, China; ²The Interventional Operation Room, Affiliated Nanhua Hospital, University of South China, Hengyang 421002, Hunan Province, China.

For correspondence:- Jianfeng Cao Email: Jianfeng586@outlook.com

Accepted: 5 January 2023 Published: 30 January 2023

Citation: Liao R, Cao J. Emodin induces osteosarcoma cell apoptosis by promoting ATM protein cleavage. Trop J Pharm Res 2023; 22(1):31-36 doi: 10.4314/tjpr.v22i1.5

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of emodin on osteosarcoma cell proliferation and apoptosis.

Methods: The osteosarcoma cell proliferation ability was determined by CCK-8, apoptosis level and cell cycle were determined via flow cytometry. Honechst staining was used to determine the ratio of nuclear pyknosis, Western blotting was used to determine the expression of AKT, p-AKT and caspase-3 precursor protein in each group of osteosarcoma cells.

Results: Emodin significantly inhibited the MG-63 cell proliferation and promoted the apoptosis of osteosarcoma cells in a dose-dependent manner (p < 0.05). It further blocked osteosarcoma cells in G1/G0 phase and decreased the expression of AKT, p-AKT, and caspase-3 (p < 0.05).

Conclusion: Emodin inhibits osteosarcoma cell growth by inhibiting ATM protein cleavage. There is a need to carry out further investigation of the effect of emodin on osteosarcoma cells using animal models in order to ascertain its potential in the management of osteosarcoma.

Keywords: Emodin, ATM, osteosarcoma, Proliferation, Apoptosis