Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Erdosteine prevents contrast-induced renal oxidative stress damage in mice

Qiwei Zhou¹, Feng Qian², Zhuo Jia¹, Xu Zhang¹

¹Department of Urology, Chinese People's Liberation Army (PLA) General Hospital/PLA Medical School, Beijing; ²Department of Emergency Medicine, Chinese People's Liberation Army (PLA) No. 92493 Hospital, Huludao, China.

For correspondence:- Xu Zhang Email: ZhangXu301@hotmail.com Tel:+861066938310

Accepted: 31 July 2021 Published: 31 August 2021

Citation: Zhou Q, Qian F, Jia Z, Zhang X. Erdosteine prevents contrast-induced renal oxidative stress damage in mice. Trop J Pharm Res 2021; 20(8):1639-1646 doi: 10.4314/tjpr.v20i8.13

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the protective effect of erdosteine on contrast-induced renal oxidative stress in mice.

Methods: C57BL/6 mice were injected intraperitoneally with contrast medium to establish an acute kidney injury model (AKI). Renal function, blood urea nitrogen (BUN) and serum creatinine (SCr) were determined. Also, oxidative stress, lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione (GSH) were evaluated. And the renal tissue structure was examined by light microscopy, while Western blot (WB) and Real-time polymerase chain reaction (RT-PCR) were used to determine the expressions of senescence-related factor, Nrf-2 and downstream antioxidant factor.

Results: Erdosteine improved the renal structure of mice and significantly decreased serum BUN and SCr levels. In addition, erdosteine promoted the expression of antioxidant enzymes SOD1, SOD2, GPX1 and GPX3 in renal tissues, decreased the content of ROS, and inhibited the content of LDH and MDA in serum. Also, WB and RT-PCR results showed that erdosteine activated Nrf2 pathway, thereby alleviating contrast-induced renal injury.

Conclusion: Erdosteine inhibits contrast-induced renal oxidative stress in mice and delays cell senescence by activating Nrf2 pathway. This will be of great significance in the treatment of contrast-induced nephropathy.

Keywords: Erdosteine, Contrast agent, Nrf-2, HO-1, Oxidative stress