Fai-Chu Wong1,2 
,
Tsun-Thai Chai1,2,
Soon-Leong Tan2,
Ann-Li Yong2
For correspondence:- Fai-Chu Wong Email: wongfc@utar.edu.my Tel:+6054688888
Received: 13 August 2012 Accepted: 15 October 2013 Published: 24 December 2013
Citation: Wong F, Chai T, Tan S, Yong A. Evaluation of Bioactivities and Phenolic Content of Selected Edible Mushrooms in Malaysia. Trop J Pharm Res 2013; 12(6):1011-1016 doi: 10.4314/tjpr.v12i6.21
© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the radical scavenging, metal chelating, antibacterial and cytotoxic activities of five edible mushrooms in Malaysia.
Methods: Mushrooms (H. tessulatus, P. eryngii, P. florida, A. polytricha and F. velutipes) were oven-dried and extracted with 90 % ethanol. Radical scavenging and metal chelating assays were based on the measurement of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferrozine absorbance at 517 and 562 nm, respectively. Mushrooms total phenolic and flavonoid contents were determined by spectrophotometric methods. Antimicrobial activity was measured using Kirby-Bauer Susceptibility Test. Cytotoxicity was assessed using brine shrimp toxicity assay.
Results: Total phenolic and flavonoid contents ranged from 0.90 - 6.03 mg GAE per g of dry sample and 0.17 - 6.95 mg QE per g of dry sample, respectively. A. polytricha demonstrated the strongest radical scavenging and metal chelating activities. Moderate antimicrobial activity was found for extracts of both P. florida and A. polytricha. Cytotoxicity LD50 ranged from 46.9 - 115.8 µg/ml.
Conclusion: The results suggest that A. polytricha is a suitable candidate for chemoprevention and would safe for use in large doses.
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