Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of anti-inflammatory and antioxidant activities of Sedum sediforme extracts

Hayat Trabsa^1,2 , Imane Krach¹, Naouel Boussoualim¹, Soraya Ouhida³, Lekhmici Arrar¹, Abderrahmane Baghiani¹

¹Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Univ. Setif, Setif 19000; ²Faculty of Exact Sciences and Nature and Life Sciences, University of Biskra, Biskra 07000; ³Faculty of Medicine, Univ. Setif, Setif 19000, Algeria.

For correspondence:- Hayat Trabsa Email: hayat.trab@yahoo.fr Tel:+213675368146

Accepted: 17 September 2020 Published: 30 October 2020

Citation: Trabsa H, Krach I, Boussoualim N, Ouhida S, Arrar L, Baghiani A. Evaluation of anti-inflammatory and antioxidant activities of Sedum sediforme extracts. Trop J Pharm Res 2020; 19(10):2109-2114 doi: 10.4314/tjpr.v19i10.14

© 2020 The authors.
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Abstract

Purpose: To evaluate the in vivo anti-inflammatory effect and in vitro antioxidant activity of the areal part of Sedum sediforme (Jacq.) extracts.

Methods: The plant was extracted with solvents of varying polarity (Methanol, chloroform and ethyl acetate, respectively) allowed its separation into three sub-fractions: crude extract, chloroform extract and ethyl acetate extract (CrE, ChE and EaE, respectively). Total polyphenol contents of the extracts were determined. The phorbolmyristate acetate (PMA) induced mice ear edema method was using to evaluate the anti-inflammatory activity. The in vitro scavenging activity was evaluated using enzymatic and non-enzymatic methods. Chelating ability of extracts was assessed using Fe²⁺–ferrozine complex.

Results: The highest content of phenolics compound was in EaE. The administration of CrE (12.5 and 25 mg/kg) reduced ear edema induced by PMA (%I = 35.81 ± 3.18 % and 38.57 ± 2.80 %, respectively), the effect was comparable with that of diclofenac used as a reference drug (%I = 38.84 ± 1.87 %). The in vitro scavenging activity of S. sediforme extracts confirmed that the CrE has the highest enzymatic and non-enzymatic activity with a half maximal inhibitory concentration (IC₅₀) = 0.063 ± 0.005 mg/mL and 0.178 ± 0.006 mg/mL, respectively. However, the ChE present an excellent chelating activity with a half-maximal effective concentration (EC₅₀) of 0.397 ± 0.001 mg/mL.

Conclusion: The results show that S. sediforme extracts have a strong antioxidant and anti-inflammatory activities which lend some support their use in the traditional medicine.

Keywords: Sedum sediforme, Anti-inflammatory, Superoxide scavenger, Cytochrome C, Metal chelating